P16蛋白表达在预测尖锐湿疣癌变中的应用  

Predicting Cancerization of Condyloma Acuminatum by Testing Expression of P16

作　　者：苏向阳[1] 赖维[1] 朱慧兰 苏祖兰[3] 郭少忠[3] 林素霞[4] 

机构地区：[1]中山大学附属第三医院皮肤性病科,广东广州510630 [2]广州市皮肤防治研究中心,广东广州510095 [3]中山大学附属第三医院病理科,广东广州510630 [4]中山大学肿瘤医院病理科,广东广州510060

出　　处：《癌症》2004年第11期1346-1349,共4页Chinese Journal of Cancer

基　　金：广东省医学科学技术研究基金项目(No.B2003048)~~

摘　　要：背景与目的:尖锐湿疣是临床上最常见的性传播疾病之一,有3%～10%的尖锐湿疣可发生癌变。目前未见关于预测尖锐湿疣癌变的报道。已发现p16基因的纯合性缺失是导致尖锐湿疣癌变的一个重要因素。本研究通过观察p16基因的表达产物———P16蛋白在尖锐湿疣及其癌变组织中的表达情况,探讨P16蛋白的异常表达与尖锐湿疣及其癌变的内在联系。方法:取30例正常皮肤(对照组)、35例尖锐湿疣和10例尖锐湿疣癌变组织,采用LSAB免疫组化法检测全部标本中P16蛋白的表达水平。结果:(1)尖锐湿疣组与正常皮肤组P16蛋白的表达以弱阳性为主,两者无显著性差异(P<0.05)。(2)湿疣癌变组P16蛋白的表达以阳性及强阳性为主,与正常皮肤组和尖锐湿疣组存在显著性差异(均P<0.01)。结论:尖锐湿疣患者P16蛋白阳性或强阳性表达提示有癌变的可能。检测P16蛋白表达是预测尖锐湿疣癌变的方法之一。BACKGROUND &OBJECTIVE: About 3%-10%of condyloma acuminatum (CA) may develop into cancer. Some studies indicated that homologous deletion of p16 gene is a major factor that causes cancerization of CA. This study was to detect expression of P16 protein in CA tissues and its cancerization tissues, and to investigate relationship of abnormal expression of P16 and cancerization of CA. METHODS: A total of 75 skin biopsy specimens were collected, including 30 normal skin samples (control group), 35 CA samples, and 10 cancerized CA samples. Expression of P16 was tested by LSAB immunohistochemistry, and relationship of P16 and cancerization of CA was statistically analyzed. RESULTS: CA and normal skin tissues showed weakly positive expression of P16, no significant difference exist (P< 0.05). Cancerized CA tissues showed positive or strongly positive expression of P16, significantly stronger than CA and normal skin tissues (P< 0.05). CONCLUSIONS: Positive and strongly positive expression of P16 in CA tissue implied risk of cancerization of CA. P16 may be a useful predictor for cancerization of CA.

关 键 词：免疫组化 尖锐湿疣 癌变 P16蛋白 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]