四川蔬菜核盘菌种群结构初步研究  

Primary studies on populational structrures of Sclerotinia sclerotiorum on vegetables in Sichuan province

作　　者：刘晓红[1] 周航[1] 姬红丽[1] 彭丽年[2] 文成敬[3] 彭云良[1] 

机构地区：[1]四川省农业科学院植物保护研究所,四川成都610066 [2]四川省农业厅植物保护站,四川成都610040 [3]四川农业大学植物保护系,四川雅安625014

出　　处：《西南农业学报》2004年第5期618-623,共6页Southwest China Journal of Agricultural Sciences

基　　金：欧盟项目(ICA4 CT2001 10054)

摘　　要：对彭州甘蓝、莴笋、菜豆、油菜4种经济作物分离所获60个菌株在含药PDA平板上测定结果表明,1μg/mL和5μg/mL多菌灵对菌丝生长抑制率平均分别为98 46%和99 08%;最低分别为93 00%和94 52%;未发现抗药性菌株,其中4个菌株的EC50均小于0 5μg/mL。27个菌株与菌丝融合群标准菌株配对后,11个菌株可以与之融合而属于7个融合群,它们分别来自莴笋(4个),甘蓝(3个),菜豆(4个)。其余16个菌株不与30个标准菌株中的任一菌株融合,此16个菌株相互两两配对后,得到11个新融合群,其中8个菌株只与自身融合。31个菌株RAPD结果表明,除甘蓝分离核盘菌与油菜分离株具有较大差异(欧氏距离>1 5)外,不同来源菌株RAPD带谱相似甚至完全相同。The measurement of sensitivity to carbendazim of 60 isolates from vegetables in Pengzhou City of Sichuan province,China,indicated no resistance in the fungal population.The EC_(50) of all of the 4 measured isolates was smaller than 0.5 μg/mL.No significant mycelial growth was observed on PDA plates amended with carbendazim.The average inhibition rate of mycelial growth of 60 isolates by carbendazim at 1 and 5 μg/ mL was 98.46 % and 99.08 %,respectively,while the minimum inhibition by carbendazim at these two concentrate was 93.00 % and 94.52 %,respectively.When paired with the 30 standard isolates of vegetative compatible groups (VCG) of Sclerotinia sclerotiorum,4 isolates from lettuce,3 from cabbage and 4 from bean out of the 28 tested isolates were compatible to standard isolates of 7 VCG from canola. The remaining 17 isolates belonged to at least 8 new VCGs of S.sclerotiorum in China.RAPD results of 31 isolates from vegetables indicated no specification of the pathogen to different hosts although isolates from the same hosts were often of same RAPD band patterns.

关 键 词：核盘菌 蔬菜 多菌灵 敏感性 菌丝融合群 RAPD 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]