复方花刺参粘多糖对家兔血管成形术后内皮功能及超微结构的影响  

The Effects of Stichopus Variegates Compound on Endothelial Function and Ultrastructure of Rabbits after Transluminal Balloon Angioplasty of Iliac Atherosclerosis Model

作　　者：王华亭[1] 蔡生业[1] 姚成芳[1] 朱宗涛[1] 王丽[1] 张维东[1] 

机构地区：[1]山东省医学科学院基础医学研究所,山东省济南市250062

出　　处：《中国动脉硬化杂志》2004年第5期497-501,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 3 0 2 71674);山东省自然科学基金 (Y2 0 0 2C43 )资助

摘　　要：为探讨复方花刺参粘多糖对成形术后血管内皮功能及超微结构的影响 ,采用光镜、扫描和透射电镜及生物化学技术观察血管成形术后血浆内皮素、血清一氧化氮浓度及血管病理形态学变化。结果发现 ,血管成形术后 4周 ,花刺参组和辛伐他汀组血浆内皮素浓度显著低于模型组 ,血清一氧化氮浓度显著高于模型组。模型组成形术段内膜厚度明显高于花刺参组和辛伐他汀组 ,管腔狭窄度明显高于花刺参组和辛伐他汀组。透射电镜观察 ,模型组成形段血管平滑肌细胞胞体肥大而致密 ,核明显增大 ,胞浆内线粒体、高尔基体、粗面内质网明显增多 ,游离核糖体增多 ,细胞内肌丝含量减少 ,呈“合成型”改变 ,花刺参组和辛伐他汀组胞体及核相对较小 ,胞浆内粗面内质网、高尔基体、线粒体较少 ,而肌丝含量相对较多 ,细胞分化程度较好 ,呈“收缩型”改变。扫描电镜观察 ,模型组血管内皮细胞排列紊乱 ,细胞联接破坏 ,有大量血小板和血液中沉积的蛋白颗粒粘附 ,花刺参组和辛伐他汀组多数血管内皮细胞排列趋于正常 ,细胞联接方式亦有所恢复 ,只有极少数血小板附着。Aim To study the effects of stichopus variegates compound on the iliac arterial endothelial function and ultrastructure of rabbits after transluminal balloon angioplasty (TBA) of iliac atherosclerosis model. Methods 50 purebred New Zealand rabbits were randomly divided into four groups: Stichopus,Simvastatin,model and normal group. Endothelium of iliac arteries in the front three groups were denuded by balloon catheter and fed with 2% cholesterol,3% lard and 3% yolk mixed forage for six weeks,and then,atherosclerotic stenosis was showed by iliac angiography. Stichopus group [0.5 g/(kg·d)] and Simvastatin group [0.5 mg/(kg·d)] were fed by gastric canal in mouth cavity. At the same time NaCl solution (0.9%) were fed in model group. All the rabbits were fed in different cages and not limited drinking. After four weeks since TBA,blood was collected from left carotid artery to measure the changes of concentrations of endothelin (ET) and nitric oxide(NO). Results ①After four weeks since TBA,the concentrations of plasm ET in model groups were higher than the one in the Simvastatin and Stichopus groups,and the concentrations of serum NO decreased obviously; the concentrations of plasm ET in Stichopus groups had an increasing tendency compared with Simvastatin and normal groups. ②After four weeks since TBA,neointimal mean thickness of segment operated TBA in model groups increased significantly,compared with Simvastatin and Stichopus groups. There were greater extent of lumen stenosis in model groups than the one in Simvastatin and Stichopus groups. ③Compared with model groups there were small vessel smooth muscle cell body and nucleus,less organelles and more myofilament contents,better cell differentiation,and less lipid droplet were phagocyted in Simvastatin and Stichopus groups under the transmission electron microscope. ④There were less platelets adherence in Simvastatin and Stichopus groups under scanning electron microscope. Conclusion Stichopus variegatus compound can significantly

关 键 词：药理学 复方花刺参粘多糖 血管成形术 再狭窄 内皮功能 内膜增殖 超微结构 兔 

分 类 号：R543.1[医药卫生—心血管疾病]