肺炎支原体膜脂蛋白诱导A549细胞mICAM-1表达上调  

Lipid Associated Membrane Protein of Mycoplasma Pneumoniae Induces the Up-regulation of Expression Level of mICAM-1 on A549 Cells

作　　者：董碧麟[1] 刘先洲[1] 汤纪路[1] 白瑞珍[1] 宗润芝[1] 

机构地区：[1]武汉大学医学院微生物学教研室,武汉430071

出　　处：《武汉大学学报（医学版）》2004年第6期642-644,651,共4页Medical Journal of Wuhan University

摘　　要：目的 :观察肺炎支原体膜脂蛋白 (Mp -LAMP)对肺腺上皮癌细胞 (A5 4 9细胞 )mICAM - 1表达的影响并探讨膜脂蛋白中的活性成分 ,为阐明肺炎支原体感染所致炎性反应奠定基础。方法 :用流式细胞术检测Mp -LAMP不同浓度刺激组、不同时间刺激组及消化酶处理组的A5 4 9细胞mICAM - 1的表达水平。结果 :与正常细胞相比 ,A5 4 9细胞受到Mp -LAMP作用后 ,mICAM - 1的表达水平明显上调 (P <0 .0 1) ,且对Mp -LAMP具有浓度依耐性。随着Mp -LAMP作用时间的延长 ,A5 4 9细胞mICAM - 1表达水平逐渐上升 ,作用 16h时达到高峰 ,然后开始下降。酶消化试验证实Mp -LAMP的活性成分是其脂质部分。 结论 :肺炎支原体膜脂蛋白中的脂质成分可以诱导A5 4 9细胞mICAM - 1表达水平的上调 ,在很大程度上影响着肺炎支原体所致炎性反应的强弱。Objective: To investigate the effect of lipid associated membrane protein of Mycoplasma pneumoniae (Mp-LAMP)on the expression of mICAM-1 on the A549 cells, to further study the target component of Mp-LAMP and to eventually lay the foundation for the explanation of inflammation caused by the infection of Mycoplasma pneumoniae.Methods: The flow cytometer assay was adopted to analyze the expression of mICAM-1 on the A549 cells respectively induced by Mp-LAMPs of different densities, of 25 μg ·ml -1 for different time and of 25 μg ·ml -1 pretreated by different enzymes including their corresponding controls.Results: The expression level of mICAM-1 on the A549 cells was enhanced obviously when incubated with Mp-LAMP as compared with that of normal cells and the extent of increase depended on the density of Mp-LAMP. The expression level of mICAM-1 increased gradually from the beginning and reached its peak at the time of 16 h then decreased slowly when incubated with Mp-LAMP of 25 μg ·ml -1. The enzyme-digestion test showed that the lipid moiety of Mp-LAMP was the target component that has the inductive ability. Conclusion: The lipid moiety of Mp-LAMP has the function to induce the upregulation of expression level of mICAM-1 on A549 cells and, to a great extent, influences the intensity of inflammation caused by the infection of Mycoplasma pneumoniae.

关 键 词：肺炎支原体 膜脂蛋白 膜结合型细胞间粘附分子-1 

分 类 号：R375[医药卫生—病原生物学]