齿密螺旋体对小鼠T细胞IL-2 mRNA表达水平及活性的影响  

Effect of Treponema Denticola on Level and Activity of IL-2 mRNA in Mice

作　　者：乔伟民[1] 乔治[2] 

机构地区：[1]武汉大学口腔医院口腔内科,武汉430079 [2]武汉大学医学院微生物学教研室,武汉430071

出　　处：《武汉大学学报（医学版）》2004年第6期649-651,共3页Medical Journal of Wuhan University

摘　　要：目的 :研究齿密螺旋体 (T .d)免疫对小鼠T细胞中IL - 2mRNA表达水平及活性的影响作用。方法 :以RT -PCR和凝胶图像吸收度分析系统检测检测T .d对小鼠脾脏T细胞IL - 2mRNA表达的影响 ;用MTT法对T细胞培养上清中IL - 2活性进行测定。结果 :T .d能在转录水平抑制静息T细胞IL - 2mRNA的表达 ,并抑制T细胞培养上清中IL - 2样活性。结论 :T .d免疫抑制作用的基础与其在转录水平抑制T细胞中IL - 2mRNA的表达有关。Objective: To understand the effect of Treponema denticola(T.d) on level and activity of IL-2 mRNA in mice. Methods: RT-PCR and gel documentation analysissystem were exploited to detect on IL-2 mRNA expression in murine. MTT were used to analyzed the activity of IL-2. Results: The level of IL-2 mRNA in rest T cell and the activity of IL-2 was inhibited by Treponema denticola(P<0.05). Conclusion: The experiments suggest that the immuno-effects of T.d may be associated with its potentiation of IL-2 mRNA expression at transcriptive level.

关 键 词：齿密螺旋体 T淋巴细胞 白细胞介素-2 

分 类 号：R542.23[医药卫生—心血管疾病] R363[医药卫生—内科学]