产物可用电喷雾质谱检测的氨基酸/肽季铵化新方法  

A Novel Method for Quaternization of Amino Acid/Peptide and Its Product Detection Using Electrospray Ionization Mass Spectrometry

作　　者：包慧敏[1] 翟建军[1] 张国安[1] 杨芃原[1] 陈先[1] 

机构地区：[1]复旦大学化学系,上海200433

出　　处：《高等学校化学学报》2004年第11期2013-2015,共3页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展计划项目(批准号:001CB510202);国家高技术研究发展计划项目(批准号:2001AA233031);国家自然科学基金(批准号:20299030)资助

摘　　要：A novel detection method for quaternization of amino acid and peptide was reported. In this method Ag 2CO 3 was employed instead of KHCO 3 to catalyze the occurrence of quaternization reaction. The absence of metal ions in the reaction system enabled us to in situ detect the quaternizing products by using electrospray ionization mass spectrometry(ESI-MS). We analyzed quaternizing products of six amino acids and two peptides in situ, and obtained the ratio of major product to by-product, which suggests the new method possesses prominent advantages over the old one. This method could offer a potential chance to combine quaternization with mass spectrometry which is helpful for the future use of quaternization reaction in quantitative proteomics.A novel detection method for quaternization of amino acid and peptide was reported. In this method Ag 2CO 3 was employed instead of KHCO 3 to catalyze the occurrence of quaternization reaction. The absence of metal ions in the reaction system enabled us to in situ detect the quaternizing products by using electrospray ionization mass spectrometry(ESI-MS). We analyzed quaternizing products of six amino acids and two peptides in situ, and obtained the ratio of major product to by-product, which suggests the new method possesses prominent advantages over the old one. This method could offer a potential chance to combine quaternization with mass spectrometry which is helpful for the future use of quaternization reaction in quantitative proteomics.

关 键 词：电喷雾离子化质谱 氨基酸/肽 季铵化 

分 类 号：O657.7[理学—分析化学]