类固醇药物对人鼻息肉组织中水通道蛋白-5表达的影响  

Effects of intranasal cortocosteroidson on the expression of Aquaporin-5 in human nasal polyps

作　　者：李志海[1] 高起学[1] 甄宏韬[1] 崔永华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉科,武汉430030

出　　处：《临床耳鼻咽喉科杂志》2004年第11期647-649,i003,共4页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :观察鼻腔局部应用丙酸氟替卡松 (FP)对鼻息肉组织中水通道蛋白 5 (AQP 5 )表达的影响 ,探讨AQP 5在鼻息肉发病中的作用。方法 :将 2 0例鼻息肉患者随机分为治疗组 (10例 )与对照组 (10例 ) ,治疗组术前给予FP喷鼻 7～ 10d ,对照组术前鼻腔未行处理。采用免疫组织化学技术检测两组鼻息肉组织AQP 5的表达情况。结果 :治疗组鼻息肉组织血管内皮AQP 5阳性细胞表达数显著降低 ,与对照组比较 ,差异有统计学意义(P <0 .0 5 ) ;而治疗组鼻息肉组织上皮细胞层及黏膜固有层腺体的AQP 5阳性细胞表达数增加 ,但与对照组比较 ,差异无统计学意义 (P >0 .0 5 )。结论 :①AQP 5可能参与鼻息肉组织水肿的形成 ;②鼻腔局部应用FP可以缩小鼻息肉体积 ,可能与AQP 5的表达改变有关 ;③研究水通道蛋白的表达与调控有助于阐明鼻息肉的发病机制和类固醇激素治疗鼻息肉的作用机制。Objective:To observe the effects of intranasal Fluticasone propionate on the expression of Aquaporin-2 in nasal polyps and explore the role of AQP-5 in the formation of nasal polyps.Method:Twenty cases of nasal polyps were selected. Fluticasone propionate were intranasally used in 10 cases of nasal polyps for 7-10 days before operation. The others,treated with nothing ,were used as control. Twenty samples nasal polyps were obtained and studied with immunochemistric technique.Result:The expression of AQP-5 positive cells in the blood vessel endothelium was statistically significantly redueced in the Fluticasone propionate-using group compared with the control group(P< 0.05).The difference of AQP-5 positive cells in mucosal epithelial and glandular epithelium was not statistically significant between the Fluticasone propionate-using group and the control group.Conclusion:①AQP-5 might be a vital factor for the formation edema of nansal polyps.② The effects of Fluticasone propionate on nasal polyps may be related to changing the expression of AQP-5 to a degree.③The full exploration of regulation and control of Aquaporions can contribute to evaluate the pathogenesis of nasal polyps.

关 键 词：鼻息肉 水通道蛋白 糖皮质激素 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]