恶性肿瘤组织中HSP70、P53表达与中医热证的关系  

Expression of HSP70 and P53 in Malignant Tumor Tissues and Its Relationship to Heat Syndrome of TCM

作　　者：王洪琦[1] 张正[1] 赵燕平[2] 李建国[1] 陈凯[3] 

机构地区：[1]广州中医药大学,广州510405 [2]华南师范大学 [3]解放军157医院

出　　处：《中国中西医结合杂志》2004年第10期897-900,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助 (No.39770 889)

摘　　要：目的探讨中医热证和非热证恶性肿瘤患者瘤组织中HSP70和P5 3的表达。方法采用S P免疫组化法进行HSP70、P5 3阳性检测以及采用ELISA法和RT PCR法检测恶性肿瘤组织中HSP70和HSP70mRNA表达含量。结果 ( 1 )结肠癌热证组、热证合计组HSP70、P5 3阳性率均明显高于各非热证组(P <0 0 5 ) ;( 2 )结肠癌热证组、鼻咽癌热证组、肺癌热证组以及热证合计组中HSP70表达含量均高于各非热证组 (P <0 0 1 ) ;( 3 )结肠癌热证组、肺癌热证组以及热证合计组中HSP70mRNA的表达含量均高于非热证组 (P <0 0 1 )。结论在恶性肿瘤的热证阶段HSP70和P5 3阳性表达率以及HSP70表达含量显著高于非热证阶段。Objective To investigate the expression of HSP70 and P53 in malignant tumor tissues of patients with TCM Heat Syndrome (HS) or with non Heat Syndrome (NHS). Methods S P immunohistochemical method was used to detect the gene expression, and ELISA and RT PCR were adopted to determine the content of HSP70 and P53 mRNA expression in different malignant tumor tissues. Results (1) Positive ratio of HSP70 and P53 in the colorectal cancer of HS was significantly higher than that in colorectal cancer of NHS respectively, and the total positive ratio of the two genes in all tumors of HS was higher than those of NHS(P<0 05); (2) HSP70 expression content in colorectal, nasopharyngeal and lung cancers of HS was higher than in those of NHS respectively, and the total HSP70 expression content in tumors of HS was higher than in those of NHS (P<0.01); (3) HSP70 mRNA expression content in colorectal and lung cancers of HS was higher than in those of NHS respectively, and the total HSP70 mRNA expression content in tumors of HS was higher than in those of NHS (P<0.01). Conclusion HSP70 and P53 positive ratio and HSP70 expression content in patients of malignant tumor with HS was higher than in those with NHS.

关 键 词：热证 HSP70 瘤组织 结肠癌 恶性肿瘤 含量 表达 结论 阶段 目的 

分 类 号：R273[医药卫生—中西医结合]