荧光光谱研究两性表面活性剂CHAPS对钝顶螺旋藻藻胆体的解离作用  

Effect of CHAPS on the Stability of Spirulina Platensis Phycobilisomes

作　　者：张熙颖[1] 刘鲁宁[1] 陈秀兰[1] 张玉忠[1] 周百成[1] 

机构地区：[1]山东大学生命科学学院

出　　处：《光谱学与光谱分析》2004年第10期1224-1226,共3页Spectroscopy and Spectral Analysis

基　　金：国家高技术研究与发展计划 ("863"计划 ) (2 0 0 2AA30 2 2 1 3);山东省科技发展计划 (0 31 0 70 1 1 3)资助项目

摘　　要：用蔗糖密度梯度高速离心的方法分离得到钝顶螺旋藻 (Spirulinaplatensis)的藻胆体 ,其室温荧光发射峰位于 6 71nm。以室温荧光光谱为表征研究了离子强度及两性表面活性剂CHAPS对藻胆体稳定性的影响。在 1mol·L-1的磷酸缓冲液中 ,藻胆体的稳定性强 ,7d内藻胆体的室温荧光发射峰位置没有变化。当用水稀释磷酸缓冲液浓度为 0 1mol·L-1,1h后藻胆体溶液的室温荧光发射峰即蓝移至 6 4 8nm ,表明藻胆体已经解离。在低浓度 (<0 6mol·L-1)磷酸缓冲液中 ,藻胆体易解离 ,解离速度随磷酸缓冲液浓度的降低而加快。在磷酸缓冲液浓度为 1mol·L-1的藻胆体的溶液中加入终浓度为 10mmol·L-1的两性表面活性剂CHAPS ,可导致藻胆体的室温荧光发射峰发生蓝移 ,说明CHAPS在高离子强度条件下也可以使藻胆体解离 。The Spirulina Platensis phycobilisomes were isolated by sucrose density gradients ultracentrifugation, and the fluorescence emission maximum of the phycobilisomes at room temperature was at 671 nm. The effects of ionic strength and the zwitterionic detergent CHAPS on the stability of the Spirulina Platensis phycobilisomes were studied by room temperature fluorescence spectrum. The phycobilisomes were stable in 1.0 mol (.) L-1 phosphate buffer solution, and their fluorescence emission maximum could remain unchanged for 7 days. The fluorescence emission maximum of phycobilisomes was blue-shifted to 648 nm when the concentration of the phosphate buffer solution was diluted to 0.1 mol (.) L-1 with deionized water, which suggested that the phycobilisomes had been dissociated. The phycobilisomes were readily dissociated in phosphate buffer solutions of low concentrations ( < 0.6 mol (.) L-1) and the speed of the dissociation increased with decreasing the concentration of the phosphate buffer solution. The fluorescence emission maximum of the phycobilisomes in 1.0 mol (.) L-1 phosphate buffer solution was blue-shifted to 648 nm when 10 mmol (.) L-1 CHAPS was added into the phycobilisomes solution, suggesting that CHAPS could dissociate phycobilisomes under high ionic strength conditions. The results might be useful for isolating intact substructures of phycobilisomes.

关 键 词：荧光光谱 两性表面活性剂CHAPS 钝顶螺旋藻 藻胆体 解离作用 分离 稳定性 

分 类 号：Q949.2[生物学—植物学]