糖尿病大鼠视网膜组织中钠钾离子三磷酸腺苷酶的变化及影响因素  

Change and influencing factor of Na^+- K^+-ATPase in retinal tissues of rats with diabetes mellitus

作　　者：杜娟[1] 李向荣[1] 黄艳霞[1] 何剑峰[1] 

机构地区：[1]柳州市人民医院眼科,广西壮族自治区柳州市545001

出　　处：《中国临床康复》2004年第32期7186-7187,共2页Chinese Journal of Clinical Rehabilitation

基　　金：广西自然科学基金资助(0007036)~~

摘　　要：目的:观察糖尿病大鼠视网膜组织中钠钾离子三磷酸腺苷酶(Na+-K+-ATPase)活性的变化,探讨糖尿病视网膜病变发生发展的有关因素。方法:用孔雀绿比色分析法对糖尿病大鼠发病后不同时期视网膜组织中Na+-K+-ATPase活性进行了检测,并与空腹血糖水平、糖基化血红蛋白含量、血脂、视网膜组织中Na+和K+含量及糖尿病病程的关系进行了分析。结果:糖尿病大鼠视网膜组织中Na+-K+-ATPase活性明显下降,K+含量下降,Na+含量增高。Na+-K+-ATPase活性与空腹血糖水平、糖基化血红蛋白含量、视网膜组织中K+和Na+含量及糖尿病病程密切相关。结论:老年糖尿病大鼠视网膜组织中Na+-K+-ATPase活性下降可能是其糖尿病视网膜病变的病理基础之一。AIM:To observe the changes of Na+ K+ ATPase activity in retinal tissue of rats with diabetic mellitus, and to explore related factors of occurrence and development of diabetic retinopathy. METHODS: Na+ K+ ATPase activity was detected with peacock green calorimetric analysis in retinal tissue of diabetic rat at various times of diabetic course, and the relativity of Na+ K+ ATPase activity with level of fasting blood glucose(FBG), hemoglobin glycosylation(HbA1c) content, blood lipid, content of K+and Na+in the retinal tissue, and the course of disease were also studied. RESULTS:Na+ K+ ATPase activity was significantly decreased, K+content decreased and Na+increased in retinal tissue of diabetic rat.There was a closed relativity among Na+ K+ ATPase activity and FBG, HbA1c, blood lipid, content of K+and Na+in retinal tissue, and course of disease. CONCLUSION: The decrease of Na+ K+ ATPase activity in retinal tissue of diabetic rat may be one of pathologic basis in occurrence of diabetic retinopathy.

关 键 词：糖尿病 大鼠 视网膜组织 钠离子 钾离子 三磷酸腺苷酶 影响因素 视网膜病变 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]