大鼠肠系膜微血管对光动力作用敏感性的研究  

Study of Mesenteric Microvascular Sensibility to PDT Effects in Rats

作　　者：张丽[1] 顾瑛[1] 刘凡光[1] 赵井泉[2] 靳玄烨[2] 

机构地区：[1]解放军总医院激光医学科,北京市100853 [2]中国科学院化学研究所

出　　处：《中国激光医学杂志》2004年第4期208-211,共4页Chinese Journal of Laser Medicine & Surgery

摘　　要：目的　比较不同分级的微血管对光动力作用组织敏感性差异 ,了解微循环因素对光动力作用敏感性的影响。方法　采用改良大鼠肠系膜活体微循环观察法 ,以Wistar大鼠为研究对象 ,采用显微数码相机录像和IMAGEPRO图像处理系统 ,选择红细胞流柱宽度百分比为评价指标 ,实时观测不同分级微血管在光动力作用中的改变及差异。结果　光动力作用时 ,微血管内皮细胞肿胀 ,红细胞聚集 ,白细胞贴壁 ,附壁血栓形成 ,管腔狭窄 ,红细胞流径减小 ,血流停滞 ,管腔闭塞。血卟啉衍生物介导光动力治疗时 ,毛细血管、微静脉、微动脉、小静脉 ,小动脉血管完全闭塞的时间分别为 6、1 6、1 8、2 2、2 6min,血卟啉单甲醚介导光动力治疗时各级微血管完全闭塞的时间为 6、1 8、30、32、4 4min ,竹红菌乙素介导光动力治疗时 ,各级微血管完全闭塞的时间为 1 6、2 6、36、4 0、5 8min。不同血管的红细胞流柱宽度百分比下降速度差异有显著意义 (P <0 0 5 )。结论　在相同的光动力条件下 ,各级微血管反应敏感性差异显著 ,由强到弱依次表现为 :毛细血管 >微静脉 >微动脉≈小静脉 >小动脉。微血管对血卟啉衍生物、血卟啉单甲醚和竹红菌乙素所介导的光动力损伤敏感性规律一致。Objective To compare the differences of microvasculature sensibility to the PDT effects in comprehending the effects of microcirculation factors on PDT Methods The in vivo mesenteric microcirculation model in rats was used in this study The PDT effects of different microvasculature were compared by intravital microscopy Results The microvascular endothelial cells swell, erythrocytes were aggregated, leucocytes were stuck to vessel wall, and mural platelet thrombi formed during PDT Emboli adhered to vessel walls and caused vascular obstruction, the diameter of the column of RBCs decreased During HpD-PDT, the stasis times of capillary, venule, arteriole, small vein and small artery were 6,16,18,22,26 min respectively;During HMME-PDT, the stasis times of microvasculatures were 6,18,30,32,44 min respectively;During HB-PDT, the stasis times of microvasculatures were 16,26,36,40,58 min respectively The differences of groups were significant( P <0 05) Conclusions PDT had the most severe injuries to capillary and the greatest influence on its RBCCD decline speed And these effects weakened in order of venule, arteriole, small vein and small artery The sensibilities of different microvasculature to PDT using HpD, HMME, HB are similar

关 键 词：微血管 完全闭塞 光动力作用 介导 肠系膜 光动力治疗 红细胞 导光 录像 图像处理系统 

分 类 号：R318.51[医药卫生—生物医学工程]