γ射线对肺成纤维细胞中Smad通路信号转导的影响及其意义  

Effects of γ rays on the signal transduction of Smad pathway in pulmonary fibro blasts

作　　者：李杨[1] 王德文[1] 宋良文[1] 彭瑞云[1] 高亚兵[1] 崔雪梅[1] 刘杰[1] 马俊杰[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2004年第5期424-426,429,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目 (3 0 170 2 92 )

摘　　要：目的检测不同剂量γ射线照射对肺成纤维细胞中Smad通路信号转导的影响并探讨其意义。方法 :进行大鼠肺成纤维细胞的原代分离培养 ,应用 3,5和 8Gyγ射线照射 ,通过免疫组织化学、免疫印迹和免疫共沉淀等方法检测照射后Smad3,4和 7蛋白的表达部位和表达水平的变化及Smad3与Smad4相互作用的变化。结果 :3,5和8Gyγ射线照射后 ,Smad3,4和 7蛋白表达水平无显著改变 ,Smad3与Smad4复合物的形成增加 ,部分大鼠肺成纤维细胞中Smad3和Smad4主要表达于细胞核。结论 :一定剂量的γ射线能促进大鼠肺成纤维细胞中Smad3与Smad4的相互结合及核转位 ,对Smad通路具有活化作用。Objective: To investigate the effects of γ irradiation on the signal transduction of Smad pathway in pulmonary fibroblasts . Methods: Rat pulmonary fibroblasts were separated, cult u red and then irradiated with 3, 5 and 8 Gy 60Co γ rays. The immunohistoc h emical localization and expression level of Smad3, 4 and 7 as well as the combi n ation of Smad3 with Smad4 were observed by means of immunohistochemistry, immun o blotting analysis and coimmunoprecipitation. Results: Afte r irradiation with 3, 5 and 8 Gy 60Co γ rays, the expression level of Sm ad3, 4 and 7 was not changed, but the combination of Smad3 and Smad4 increased . At the same time, the expressions of Smad3 and Smad4 proteins in some fibroblas ts translocated to nucleus. Conclusion: A certain dose of 60Co γ rays may promote the combination and nuclear translocation of Sm ad 3 and Smad4,indicating that γ rays may activate Smad signaling pathway in pulm onary fibroblasts.

关 键 词：SMAD信号通路 肺成纤维细胞 转化生长因子Β 信号转导 肺纤维化 辐射剂量 

分 类 号：R818[医药卫生—放射医学]