瓣下心肌可增强大鼠同种带瓣主动脉移植物免疫原性  

Adhering muscle cuff in rat aortic valve allograft enhances graft immunogenicity

作　　者：陈艰[1] 于建华[2] 刘江[1] 徐全[1] 谢爱民[1] 李守先[2] 

机构地区：[1]江西省人民医院心胸外科,南昌330006 [2]山东医科大学附属医院心脏外科,济南250012

出　　处：《基础医学与临床》2004年第5期560-562,共3页Basic and Clinical Medicine

摘　　要：对大鼠同种带瓣主动脉 (AVH)不同组织 (瓣下心肌、瓣叶、大动脉壁 )免疫原性进行比较 ,观察AVH移植后机体免疫排斥反应。采用大鼠AVH腹主动脉异位移植模型。实验分 3组 ,分别接受带瓣下心肌的AVH (A组 ,n =12 )、去除瓣下心肌的AVH(B组 ,n =12 )和单纯主动脉壁 (C组 ,n =12 )移植 ,以术前为对照。术后 2、4、8、12周测定血ICAM 1、TCR αβ、移植标本CD4 0 /CD4 0L的表达 ,并行组织病理学观察。 3组术后ICAM 1、TCR αβ和CD4 0 /CD4 0L的表达均比对照组明显增高 (P <0 0 5 )。A组术后 2～ 4周ICAM 1分别为 (9 0 32± 2 5 82 ) %和 (17 0 6±2 6 4 6 ) % ,明显高于B、C 2组 (P <0 0 1) ;术后 2周TCR αβ为 (4 1 5 9± 5 96 5 ) % ,亦明显高于B、C 2组 (P <0 0 5 ) ;术后 2周CD4 0表达为 4 333± 0 5 77,与C组相比差异明显 (P <0 0 5 )。病理学观察 3组均呈不同程度的免疫排斥反应表现 ,B、C 2组改变相似 ,A组病理改变比B、C 2组出现早、程度重。大鼠AVH移植诱发了机体免疫排斥反应 ,与瓣叶和动脉壁相比 。To study the antigenicity of different tissues in rat aortic valve allograft. AVHs were heterotopically allografted into abdominal aorta from Wistar to SD rats.According to different donor tissue types,recipients were divided into three groups:group A received a graft involving aortic wall,leaflet and adhering muscle cuff(n=12);group B received aortic wall and leaflet(n=12); group C received only aortic wall(n=12).Animais(n=3)received non-implantation acted as the controls.Expressions of ICAM-1,TCR-αβ,CD40/40L were tested and histological significant increases of ICAM-1,TCR-αβ in serum and CD40/40L expression of explants at the 2nd and 4th week postimplantatively were seen in all 3 groups. The expression of ICAM-1 was (9 032±2 582)% and (17 06±2 646)% respectively at the 2nd and 4th week in group A,and was higher than that in group B and group C (P<0 05).The expression of CD40 in group A was markedly enhanced as compared with that in group C at the postoperative 2nd week(P<0 05).Histological study showed typical appearance of rejection in all three groups.However,the extent of histological changes was more serious in group A than in group B and C.Compared with leaflet or aortic wall,adhering muscle cuff had relative higher efficacy of immunogenicity.

关 键 词：同种异体移植 同种带瓣主动脉 免疫原性 大鼠 

分 类 号：R654.2[医药卫生—外科学]