TLSF_(JM)在体内对同种异体抗原诱导的小鼠T细胞增殖和杀伤细胞分化的影响  

The effect of TLSF_(JM) on alloantigen-induced diff erentiation and cytotoxicity of mouse CTL

作　　者：韩卫宁[1] 董邦权[1] 李琦[1] 曹云新[1] 张赟[1] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室,西安710032

出　　处：《中国免疫学杂志》2004年第11期768-771,共4页Chinese Journal of Immunology

摘　　要：目的 :探讨JM急性T淋巴细胞白血病细胞分泌的抑制因子 (TLSFJM)对BALB C小鼠胸腺发育的影响及其在体内对同种异体抗原诱导的小鼠辅助性T细胞 (Th)增殖及杀伤性T细胞 (CTL)分化的影响。方法 :建立小鼠同种异基因型肿瘤排斥模型 ,实验小鼠腹腔注射含TLSFJM 的JM细胞培养上清 ,对照小鼠注射RPMI16 4 0。无菌取小鼠脾脏细胞 ,分别以3H TdR掺入法和51 Cr释放法检测T细胞增殖和CTL的分化。结果 :①TLSFJM 明显抑制多克隆刺激剂PHA联合IL 2诱导的小鼠胸腺细胞增殖 ;②体内注射TLSFJM明显抑制多克隆刺激剂PHA联合IL 2诱导的小鼠T细胞活化、增殖 ;③TLSFJM在体内能有效抑制同种异体抗原诱导的小鼠T细胞增殖 ;④TLSFJM 在体内能有效抑制同种异体抗原诱导的小鼠CTL分化。结论 :TLSFJM 在体内能有效抑制同种异体抗原诱导的小鼠T细胞增殖和小鼠CTL分化。Objective:To investigate the influence of JM acute T leukemia cell line derived suppressor factor(TLSF JM) on the thymocyte development of BALB/C mice and i n vivo effect of TLSF JM on alloantigen-induced Th proliferation and CT L differentiation.Methods:Mouse allogenic tumour regection model was set up, and the mice were injected with JM cell supernatant containing TLSF JM or R PMI1640.The splenocytes were isolated,Th proliferation and CTL differentiation w ere tested by 3H-TdR incorporation and 51Cr release method resp ectively.Results:①TLSF JM markedly suppressed PHA plus IL-2-induced proliferation of BA LB/C thymocyte;②Peritoneolly injected TLSF JM clearly inhibited P HA plus IL-2-induced T proliferation;③TLSF JM markedly suppressed allo antigen-induced proliferation of T cells in vivo;④TLSF JM markedly suppressed alloantigen-induced differentiation of CTL in vivo.Conclusion:TLSF JM could markedly suppress alloantige n-induced T cells proliferation and CTL differentiation in vivo. [

关 键 词：抑制因子 CTL 分化 

分 类 号：R392.11[医药卫生—免疫学]