β-catenin在大鼠毛囊损伤修复中表达变化的研究  

A study of expression of P-catenin in rat hair follicle wound repair

作　　者：张艺[1] 杨恬[1] 曾益军[1] 

机构地区：[1]第三军医大学基础部细胞生物学教研室,重庆400038

出　　处：《创伤外科杂志》2004年第6期448-450,共3页Journal of Traumatic Surgery

基　　金：国家自然科学基金项目(No:30371383)

摘　　要：目的 探讨大鼠毛囊损伤修复过程中β连环蛋白(β-catenin)表达的变化规律及意义。方法 建立大鼠触须毛囊损伤动物模型,应用免疫组化以及RT-PCR检测大鼠毛囊损伤前后β-catenin表达的变化,以及损伤后不同时期β-catenin mRNA水平的改变。结果 正常大鼠毛囊中β-catenin仅有微弱表达,而损伤后毛囊外根鞘细胞中强表达β-catenin;RT-PCR结果表明β-catenin mRNA在毛囊损伤后5小时明显上升,在损伤24小时后达到峰值,随后逐渐下降,到72小时时恢复正常水平。结论 β-catenin在大鼠毛囊损伤前后表达明显改变,提示β-catenin在大鼠毛囊损伤修复中起重要作用,可能是大鼠毛囊生长、周期维持的重要调节因子。Objective To explore the expression of β-catenin in the process of hair follicle wound repair in rats. Methods On the basis of a rat wound model, IHC and RT-PCR were used to detect the expression of β-catenin in wound hair follicle and normal hair follicle tissue. Results It showed that the expression ofβ-catenin was stronger in wound hair follicle than that of normal hair follicle tissue; and the transcription level of β-catenin was associated with the wound stages. RT-PCR showed that β-catenin mRNA increased rapidly after 5h,it reached peak level after 24h|decreased slowly and then reached normal level after 72h. Conclusion The expression ofβ-catenin was altered obviously in RAT wound hair follicles. It suggested that β-catenin might be an essential regulative factor in follicle formation and cycle maintenance, and plays an important role in rat hair follicles wound repair.

关 键 词：毛囊 损伤修复 Β-CATENIN 

分 类 号：R322.995[医药卫生—人体解剖和组织胚胎学] R329.33[医药卫生—基础医学]