日本血吸虫病患者γ干扰素及其受体检测意义  

The Clinical Significance of Interferon-γ and its Receptor in Patients with Schistosomiasis

作　　者：邵平扬[1] 蔡卫民[2] 丁韧烨[1] 乌文琳[1] 黄育英[1] 

机构地区：[1]浙江省嘉兴市第一医院,嘉兴314000 [2]浙江大学附属第一医院传染病研究所,杭州310003

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第5期271-273,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的　研究γ干扰素 (IFN γ)及I型γ干扰素受体 (IFN γR1)在慢性血吸虫病 (慢血 )及晚期血吸虫病 (晚血 )患者中表达水平 ,探讨IFN γ和IFN γR1表达的临床意义及其和肝纤维化的关系。　方法　用流式细胞术检测慢血及晚血患者外周血淋巴细胞IFN γR1的表达水平 ;用ELISA法检测慢血及晚血患者血清IFN γ水平 ;用放射免疫法检测血清中透明质酸 (HA)、Ⅳ胶原 (Ⅳ C)、层粘连蛋白 (LN)、Ⅲ型前胶原 (PCⅢ )等肝纤维化指标。采用对比分析 ,观察IFN γR1、IFN γ及肝纤维化指标在慢血及晚血患者中的表达水平及相关性。　结果　慢血患者IFN γR1和IFN γ与健康对照组比较差异无显著性 (P >0 .0 5 ) ;晚血未切脾患者IFN γR1表达明显低于其他组 (P <0 .0 5 ) ,而IFN γ增高 (P <0 .0 1) ;晚血切脾患者此两项指标又恢复正常 ;IFN γR1和IFN γ与HA、Ⅳ C、LN、PCⅢ之间无相关性 (r分别为 0 .19、0 .2 0、0 .14、0 .2 1)。结论　IFN γR1和IFN γ之间存在相互对应关系 ,IFNObjective To study the expression of interferon γ(IFN γ) and typeⅠinterferon γ receptor (IFN γR1) in the chronic and advanced patients of schistosomiasis japonica, and to discuss its clinical relationship with hepatic fibrosis. Methods IFN γR1 in the peripheral blood lymphocytes was detected by ELISA, and the hyaluronic acid (HA), collagen type Ⅳ(C Ⅳ), procollagen type Ⅲ (PCⅢ), laminin (LN) were detected by radio immunoassay (RIA) in schistosomiasis patients. The level of IFN γ, IFN γR1 and serum markers of hepatic fibrosis were observed, and the relationship with each other was analyzed by statistical method. Results There was no difference in the expression of IFN γ and IFN γR1 between the patients with chronic schistosomiasis and the normal group ( P >0.05), the IFN γR1 in advanced cases without splenectomy was low ( P <0.05), but IFN γ was high( P <0.01). The two indicators in the advanced schistosomiasis patients with splenectomy returned to normal. There was no corresponding relationship between the two indicators and HA, C Ⅳ,PCⅢ,LN with a r value of 0.19, 0.20, 0.14, and 0.21 respectively. Conclusion There is a corresponding relationship between IFN γ and IFN γR1; the expression of IFN γR1 is related to the course of schistosomiasis, and the relationship with hepatic fibrosis needs further study.

关 键 词：IFN-γ 患者 Γ干扰素 受体 表达水平 肝纤维化指标 切脾 相关性 日本血吸虫病 病程 

分 类 号：R532.21[医药卫生—内科学] R512[医药卫生—临床医学]