MP-1对脓毒症小鼠的保护作用及其机制  

Effect and mechanism of mastoparan-1 protecting septic mice

作　　者：郭毅斌[1] 郑江[1] 吕根法[1] 卫国[1] 

机构地区：[1]第三军医大学附属西南医院综合实验研究中心,重庆400038

出　　处：《中华创伤杂志》2004年第11期678-681,共4页Chinese Journal of Trauma

基　　金：国家重点基础研究发展规划资助项目(G1999054203)

摘　　要：目的观察阳离子多肽MP-1对脓毒症小鼠腹腔巨噬细胞(PMφ)呼吸爆发的影响。方法观察MP-1(3mg/kg)对内毒素(LPS)(20mg/kg)攻击的脓毒症模型小鼠的保护作用;体外培养小鼠PMφ,将MP-1与PMφ在37℃下孵育2h后,用LPS400ng/ml刺激PMφ,采用紫外分光光度法检测并换算得到超氧阴离子(O-2)的量和还原型辅酶Ⅱ(NADPH)氧化酶的活性,荧光法检测过氧化氢(H2O2)浓度。结果MP-1可显著抑制LPS刺激小鼠PMφ产生O-2、H2O2及细胞膜上NADPH氧化酶的活性。结论MP-1对LPS攻击的小鼠有显著的保护作用,其机制可能与MP-1对脓毒症小鼠巨噬细胞呼吸爆发的抑制作用有关。Objective To observe the influence of cationic peptide mastoparan 1 (MP 1) on respiratory burst of murine peritoneal macrophages (PMφ) stimulated by lipopolysaccharide (LPS). Methods The effect of MP 1 (3 mg/kg) protecting mice challenged by lethal LPS (20 mg/kg) was observed by means that the murine PMφ was preincubated with MP 1 for two hours in vitro before the murine PMφ was stimulated with LPS (400 ng/ml). The superoxide anion and the activity of NADPH oxidase in PMφ were assayed by fluorescence spectrophotometer and the hydrogen peroxide released from PMφ measured by ultraviolet spectrophotometer. Results MP 1 could significantly protect mice from LPS challenging and inhibit the value of superoxide anion and hydrogen peroxide as well as the activity of NADPH oxidase in PMφ. Conclusions MP 1 can exert significantly protective effect on the mice challenged by lethal LPS, as may relate to the inhibitive effect of MP 1 on the respiratory burst in macrophage of the septic mice.

关 键 词：MP PMΦ 小鼠 脓毒症 保护作用 LPS 呼吸爆发 辅酶 NADPH氧化酶 超氧阴离子 

分 类 号：R96[医药卫生—药理学]