中华眼镜蛇毒活性组分体外抑制三维血管生成的实验研究  

Inhibition of active fraction separated from Naja Atra venom on three dimensional angiogenesis

作　　者：余清声[1] 刘晓颖[2] 覃媛[1] 黄劭[1] 

机构地区：[1]广州医学院广州蛇毒研究所,广州510182 [2]广东省人民医院心血管病研究所

出　　处：《中国临床药理学与治疗学》2004年第10期1105-1109,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广东省自然科学基金项目(№980463);卫生部科技基金课题(№982349)

摘　　要：目的研究中华眼镜蛇毒活性组分的抗血管生成作用。方法采用拟血管生成三维培养法,观察活性组分对体外血管生成的抑制效应。结果活性组分可抑制内皮细胞在培养基质中生成血管网状三维结构的反应,0.6、1.2、2.4μg·ml-1的不同浓度组分抑制程度不同。在0.6μg·ml-1浓度组,培养基质Matrigel中的内皮细胞团只形成局部的、不完整的空间网状结构;在1.2μg·ml-1浓度组,悬浮于凝胶中的细胞团所形成的管芽不能相互连接而形成空间网状结构;在2.4μg·ml-1浓度组,细胞散在悬浮于胶层中大部分不能粘聚,且随培养时间的延长无明显的结构变化。结论中华眼镜蛇毒活性组分具有体外抑制血管生成的生物活性。AIM: To study the anti angiogenesis effects of the active fraction separated from Naja Atra venom. METHODS: The inhibiting effect of active fraction in angiogenesis was detected by the three dimensional angiogenesis model. RESULES: The active fraction in different concentrations inhibit the action that vascular endothelial cells (ECs) developed into three dimensional capillary like networks in vivo. ECs in matrigel only developed into incomplete capillary like networks in 0.6 μg·ml -1 dose group, the sprouts differentiated from ECs did not spread and contract each other and form the nets in 1.2 mg·L -1 dose group, ECs did not adherent together and alternate in 2.4 mg·L -1 dose group. CONCLUSION: The active fraction separated from Naja Atra venom has the anti angiogenesis effect in in vitro.

关 键 词：中华眼镜蛇毒 抗血管生成 三维血管生 成 内皮细胞 体外 细胞培养 

分 类 号：R966[医药卫生—药理学]