缬沙坦和螺内酯对高血压大鼠心肌胰岛素样生长因子-1的作用  

Effects of valsartan and spironolactone on insulin like growth factor-1 in cardiacmyocytes of spontaneously hypertensive rats

作　　者：段小春[1] 王晋明[1] 王芳[1] 李恒[1] 秦晋梅[1] 

机构地区：[1]武汉大学人民医院心血管内科

出　　处：《中国临床药理学与治疗学》2004年第10期1137-1141,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖北省教育厅自然基金项目(№2000B02303;№301140080)

摘　　要：目的观察自发性高血压大鼠(SHR)和WKY大鼠心肌胰岛素样生长因子1(IGF1)的浓度和表达及缬沙坦与螺内酯对SHRIGF1的影响,探讨IGF1与心肌肥厚的病理机制。方法将18只6周龄SHR随机分成3组,每组6只。其中2组分别灌胃缬沙坦30mg·kg-1·d-1和螺内酯20mg·kg-1·d-1,SHR阳性对照组给正常饮水,并与雄性同周龄WKY大鼠比较。实验期为13周,放射免疫法检测心肌IGF1的浓度,免疫组化法检测4组大鼠心肌IGF1的表达。结果SHR对照组IGF1表达和浓度均明显高于WKY组(P<0.01),缬沙坦组和螺内酯组的IGF1较SHR对照组下降(P<0.01),而且缬沙坦组比螺内酯组下降的更明显(P<0.05),两组血压、LVMBW均显著低于SHR组(P<0.01)。结论IGF1在肥厚心肌中表达明显增加,而缬沙坦和螺内酯在降压的同时可以抑制IGF1的表达,提示IGF1在心肌肥厚发展中起一定作用。AIM: To study the inhibitive effects of valsartan and spironolactone on expression of insulin like growth factor 1 in cardiacmyocytes of spontaneously hypertensive rats. METHODS: 18 SHRs were randomly divided into 3 groups (n=6 in each): valsartan group treated with 30 mg·kg -1 ·d -1 valsartan, spironolactone group treated with 20 mg·kg -1 ·d -1 spironolactone, and control group treated with placebo. All were administrated by gastric perfusion. 6 WKY rats were served as control. After 13 weeks administration, the expressions of IGF 1 in cardiacmycytes were measured by immunohistochemistry and the IGF 1 concentrations in myocardium were measured by radiommunoassay. RESULTS: The expression of IGF 1 was higher in SHR group than that in the WKY group (P< 0.01 ). Valsartan and spironolactone inhibited the expression of IGF 1. The inhibitive effects of IGF 1 in valsartan group were more significantly higher than those in spironolactone group. Valsartan and spironolactone decreased blood pressure and left ventricular mass to body weight ratio (LVM/BW). CONCLUSION: IGF 1 may play an important role in the development of cardiac hypertrophy in SHR. Valsartan and spironolactone treatment can inhibit the expression of IGF 1 and prophylaxi myocardial hypertrophy.

关 键 词：高血压 心肌肥厚 胰岛素样生长因子-1 缬沙坦 螺内酯 

分 类 号：R972.4[医药卫生—药品]