重型再生障碍性贫血患者骨髓Ⅰ型树突细胞亚群的变化  

Changes of subsets of DC 1 in the bone marrow of severe aplastic anemia patients

作　　者：何广胜[1,2] 邵宗鸿[1] 和虹[1] 刘鸿[1] 付蓉[1] 白洁[1] 施均[1] 曹燕然[1] 涂梅峰[1] 孙娟[1] 贾海荣 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,血液病医院,天津现在300020 [2]苏州大学附属第一医院江苏省血液病研究所

出　　处：《中华血液学杂志》2004年第11期649-652,共4页Chinese Journal of Hematology

基　　金：天津市自然科学基金资助项目 (0 0 3 60 611)

摘　　要：目的　测定重型再生障碍性贫血 (SAA)患者在免疫抑制治疗前、后骨髓中Ⅰ型树突细胞(DC1)亚群 (CD11c+ CD1a+ 细胞、CD11c+ CD83+ 细胞 )的改变 ,评价CD11c+ CD83+ 细胞与Th1细胞、CD3+ CD8+ 细胞及造血功能的相关性 ,探讨DC1在SAA发病机制中的作用。方法　以正常人为对照 ,用单克隆抗体及流式细胞仪检测发病期和恢复期SAA患者骨髓中Th1细胞、CD3+ CD8+ 细胞、CD11c+ CD1a+ 细胞、CD11c+ CD83+ 细胞百分率及CD11c+ CD83+ 细胞 /CD11c+ CD1a+ 细胞比值的改变 ;评价CD11c+ CD83+ 细胞与Th1细胞、CD3+ CD8+ 细胞及网织红细胞绝对值、中性粒细胞绝对值(ANC)的相关性。结果　正常人骨髓中Th1细胞、CD11c+ CD1a+ 细胞、CD11c+ CD83+ 细胞百分率及CD11c+ CD83+ 细胞 /CD1a+ CD11c+ 细胞比值分别为 (0 .4 2± 0 .30 ) %、(0 .38± 0 .2 9) %、(0 .37±0 .32 ) %、1.0 7± 0 .10 ,SAA患者发病期为 (4.87± 0 .5 4 ) %、(1.73± 0 .2 4 ) %、(3.38± 0 .5 6 ) %、2 .2 1±0 .32 ,SAA患者恢复期为 (0 .5 3± 0 .2 2 ) %、(0 .6 1± 0 .2 3) %、(0 .6 5± 0 .2 2 ) %、1.37± 0 .2 5 ;SAA患者发病期Th1细胞、CD11c+ CD1a+ 细胞、CD11c+ CD83+ 细胞百分率及CD11c+ CD83+ 细胞 /CD11c+CD1a+Objective To measure the subsets of dendritic cells 1 (DC1) in the bone marrow of severe aplastic anemia (SAA) patients and evaluate the relationships between the CD11c +CD83 +cells and Th1 cells, CD3 +CD8 + cells or hematopoietic function and explore the role of DC1 in the pathogenesis of SAA. Methods By FACS, the quantities and ratios of CD11c +CD1a + cells, CD11c +CD83 + cells, Th1 cells, and CD3 +CD8 + cells in the bone marrow of SAA patients and normal controls were detected respectively. The relationships between CD3 +CD8 + cells and reticulocyte absolute value(Ret) or neutrophil absolute value (ANC), between Th1 cells and CD3 +CD8 + cells, Ret or ANC, between CD11c +CD83 + cells, and Th1 cells, CD3 +CD8 + cells, Ret or ANC were evaluated. Results In normal control’s bone marrow, the percentages of Th1 cells, CD11c +CD1a + cells, CD11c +CD83 + cells and the ratio of CD11c +CD83 + / CD11c +CD1a + were (0.42±0.30)%,(0.38±0.29)%, (0.37±0.32)% and 1.07±0.10, respectively. In untreated SAA patients, they were (4.87±0.54)%,(1.73±0.24)%,(3.38±0.56)% and 2.21±0.32 respectively,which were higher than that in mornal controls (P<0.01). In recovering SAA patients, the percentages of Th1 cells, CD11c +CD1a + cells and CD11c +CD83 + cells decreased significantly to(0.53± 0.22 )%,(0.61±0.23)%,(0.65±0.22)%, respectively (P<0.01). The ratio of CD11c +CD83 +/ CD11c + CD1a + in recovering SAA patients decreased to 1.37±0.25, which was similar to that in normal controls (P>0.05). The percentage of CD3 +CD8 + cells in untreated SAA patients was(32.32± 10.22 )%, and in recovering SAA patients decreased to (13.67±5.24)% (P<0.01). The percentage of CD3 +CD8 + cells in SAA patients was negatively correlated with their Ret and ANC (P<0.05), while their Th1 cell percentages were positively correlated with their CD3 +CD8 + cells (P<0.01), and negatively correlated with their Ret and ANC (P<0.01). SAA patient’s CD11c +CD83 + cell percentages were positively correlat

关 键 词：CD1A Th1细胞 CD83 患者 CD3^+ CD8^+细胞 骨髓 SAA 负相关 百分率 

分 类 号：R556.5[医药卫生—血液循环系统疾病]