HPLC-ELSD测定芪休颗粒中黄芪甲苷的含量  

Determination of astragaloside Ⅳ in Qixiu Granules by HPLC-ELSD

作　　者：田景奎[1] 吴丽敏[2] 刘建建[3] 王爱武[2] 耿晖[2] 刘洪梅[2] 

机构地区：[1]浙江大学药学院,浙江杭州310027 [2]山东省立医院天然药物研究中心,山东济南250021 [3]山东大学药学院,山东济南250002

出　　处：《中成药》2004年第10期812-814,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :制定芪休颗粒 (黄芪、丹参、赤芍等 )中黄芪甲苷的含量测定方法。方法 :用HPLC ELSD法 ,AlltimaC1 8色谱柱 ,流动相乙腈 水 (34∶6 6 ) ,流速 1mL·min-1 ;ELSD参数 :漂移管温度 1 0 0°C ,气流量 2 .7L·min-1 (压缩空气 )。结果 :线性范围为 3.73～ 1 3.5 9μg ,回归方程为Y =- 4 .4 0× 1 0 5+2 .0 7× 1 0 5X ,r =0 .9992 ,回收率为 1 0 0 .73%,RSD为 3.97%。结论 :本研究建立的方法简便、可靠、准确 ,可以作为芪休颗粒的质量控制方法。AIM: To determine astragaloside Ⅳ in Qixiu Granule (Radix Astragali seu Hedysari, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, etc.) by HPLC ELSD. METHODS: HPLC ELSD was used in the quantitative analysis by using Alltima C 18 chromatography column and acetonitril water (34∶66) as a mobile phase. The flow rate of mobile phase was 1mL·min -1 . The tube temperature of the detector was 100°C. The flow rate of pure air was 2.7L·min -1 . RESULTS: The regressione quation was Y=-4.40×105+2.07×105X, r=0.9992 (3.73～13.59μg). The average recovery of astragaloside Ⅳ was 100.73%, RSD was 3.97%. CONCLUSION: The method is simple, reliable, accurate and can be regard as the quantity control method of Qixiu Granule.

关 键 词：芪休颗粒 HPLC-ELSD 黄芪甲苷 

分 类 号：R927.2[医药卫生—药学]