姜黄素抗大鼠肝星状细胞氧应激脂质过氧化作用的研究  

Effect of curcumin on lipid peroxidation of oxidative stress in rat stellate cell of liver

作　　者：刘永刚[1] 刘永忠[2] 王晓东[3] 

机构地区：[1]武警广东总队医院药剂科,广东广州510517 [2]西安市中心医院药剂科,陕西西安710003 [3]广州市花城制药厂,广东广州510555

出　　处：《中成药》2004年第10期829-832,共4页Chinese Traditional Patent Medicine

摘　　要：目的 :探讨姜黄素 (Curcumin ,Cur)对大鼠肝星状细胞 (hepaticstellatecells,HSC)氧应激所致脂质过氧化的作用。方法 :HSC与FeNTA共同培养产生氧应激。以MTT法检测姜黄素对HSC增殖的效应 ,LDH法检测姜黄素对HSC的毒性作用 ,以ELISA法测定细胞培养液中Ⅰ型胶原的水平 ,以试剂盒分别测定细胞培养液中MDA、GSH、GSH PX、SOD水平。结果 :HSC与FeNTA共同培养 ,细胞内MDA、GSH水平明显升高 ,GSH PX、SOD活力明显降低。FeNTA与HSC共同培养 ,能明显促进HSC增殖和提高细胞培养上清中Ⅰ型胶原的水平。姜黄素可以逆转上述所有FeNTA所致效应。结论 :姜黄素抗肝纤维化作用可能与其抗脂质过氧化作用有关。AIM: To investigate the effect of antioxidant curcumin on the lipid peroxidation of oxidative stress in rat hepatic stellate cell in vitro. METHODS: HSC T6 were incubated with 0.1mM ferric nitrilotriacetate complex (FeNTA), MTT colorimetry was used for assaying proliferation of HSC, Cytotoxicity was measured by LDH colorimetry, Collagen type Ⅰ accummulation in the culture media was measured by ELISA, Intracellular malonildialdehyde (MDA)、SOD、GSH and GSH PX levels in the culture media were measured by their reagent boxes. RESULTS: HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decrease SOD and GSH PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type Ⅰ levels in the culture medium. All these effects were reversed by the antioxidant curcumin. CONCLUSION: The mechanism of curcumin on fibrosis may be related to inhibit lipid peroxidation

关 键 词：姜黄素 肝星状细胞 氧应激 纤维化 

分 类 号：R285.5[医药卫生—中药学]