人角蛋白17的HLA-DR限制性T细胞表位区的确定  

Identification of HLA-DR-restricted T cell epitope regions on keratin 17

作　　者：沈柱[1] 王刚[1] 樊建勇[1] 李巍[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院皮肤科,西安710032

出　　处：《中华微生物学和免疫学杂志》2004年第10期769-772,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目(No.30371650);第四军医大学创新工程课题(No.CX02A011);第四军医大学优秀博士课题资助(No.2003004)

摘　　要：目的　对银屑病自身抗原———角蛋白 17的HLA DR限制性T细胞表位区进行确定。方法　借助相关软件和互联网服务器进行角蛋白 17的辅助性T细胞抗原表位区预测 ;从银屑病患者皮损组织中扩增出人角蛋白 17全长基因 ;根据预测结果 ,融合表达、纯化相应的表位区 ;分离纯化外周血T淋巴细胞 ,将各表位区肽段分别与正常人和银屑病患者的T淋巴细胞体外作用 ,检测T细胞增殖程度和培养上清中IFN γ水平变化 ,从而筛选出特异性强反应性表位区。结果　角蛋白 17的 6 5～12 0肽段、30 5～ 374肽段能够显著促进银屑病患者T淋巴细胞增殖和IFN γ分泌 ,与正常对照组比较差异有非常显著性 (均为P <0 .0 0 1)。结论　人角蛋白 17的 6 5～ 12 0肽段和 30 5～ 374肽段是银屑病特异性强反应性HLA DR限制性T细胞表位区。本研究为以后研究银屑病的免疫发病机制和治疗打下了一定基础。Objective To identify the HLA-DR-restricted T cell epitope regions on keratin 17 (K17), one of the autoantigens of psoriasis. Methods Epitope prediction was based on related softwares and internet servers. K17 gene was amplified from epidermis of psoriatic patients and the predicted epitopes were expressed and purified. T cells were isolated and purified and were detected as the level of proliferation and the concentration of IFN-γ in the culture. Results Compared with the control group and other regions, 65-120 and 305-374 regions of human K17 had a positive role on the proliferation and IFN-γ expression of T cells from psoriatic patients(P< 0.001). Conclusion The results indicated that 65-120 and 305-374 peptide regions are the psoriasis-specific HLA-DR-restricted T cell epitope regions. The advanced study based on these peptide regions can provide a more complete understanding of the immunological basis of the disease.[

关 键 词：角蛋白17 银屑病 T细胞表位 患者 HLA-DR HLA—DR 治疗 融合表达 分离纯化 限制性 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]