贝克曼库尔特LH750血液分析仪白细胞分类计数应用评价  

The Evaluation of Differential Count by Beckman Coulter LH 750 and its Application

作　　者：韩丽君[1] 费选文[1] 黄宇泽[1] 朱红军[1] 陈书裕[1] 

机构地区：[1]汕头市中心医院,广东汕头515031

出　　处：《实用医技杂志》2004年第10B期2140-2142,共3页Journal of Practical Medical Techniques

摘　　要：目的 :探讨贝克曼库尔特 L H75 0血液分析仪的白细胞分类计数等有核细胞检测的准确性。方法 :用同份标本分别在 MAXM型号和 L H75 0型号仪器上做重复性 (CV% )的对比 ;选取 30份标本同时做血涂片的显微镜分类计数和嗜酸性粒细胞、单核细胞二项的直接计数 ,结果用统计学处理 ,观察与仪器分类结果的各类型细胞的各自的相关性 ;对仪器检测的有核红细胞的结果和怀疑性提示做了初步的复查证实。结果 :L H 75 0型号的重复性优于MAXM型号 ;与血涂片的显微镜分类计数相关性好 ,嗜酸性粒细胞、单核细胞二项与直接计数的相关性明显提高 ,分别为 0 .92 5和 0 .86 4 ;有核红细胞的符合率为 87.5 % ;有未成熟白细胞等怀疑性的提示 ,绝大多数可在显微镜下得到确认。结论 :L H 75 0血液分析仪的白细胞分类相对于同厂家的其他型号的仪器准确性好 ,选择该仪器可信任血常规的过筛工作 。Objectives To evaluate the accuracy of the nucleated cells count w hich including differential count by Coulter LH 750. Methods Comp ared the repea tedly of differential count by Coulter LH 750 and Coulter MAXM. Counted eosinop hil and mononuclear and differential count of 30 blood samples by microscope, Ob served the correlation of differential count by microscope and Coulter LH 750 by statistics analysis. When have clue of erythroblast cells by Coulter LH 750,id entified it by microscope examination. Results The repeatedly of C oulter LH 750 is higher than Coulter MAXM. There were perfect correlation of differential coun t between by microscope count and LH 750, and the correlation of eosinophil and mononuclear count had raised (r=0.925 and 0.864), the coincidence ratio of eryth roblast was 87.5 %. The mass of clue of abnormal nucleated cells by LH 750 can b e en identified by microscope examination. Conclusions The accuracy of differenti al count by LH 750 is higher than others Coulter blood cell analysor. LH 750 is effective in screening abnormal nucleated cells, and it must been identified by microscope examination.

关 键 词：LH 血液分析仪 白细胞分类计数 显微镜 血涂片 直接计数 标本 确认 查证 厂家 

分 类 号：R446.1[医药卫生—诊断学]