红景天属4种植物RAPD分析与分类鉴定  

Identification of four Rhodiola L. plants by RAPD

作　　者：胡挺松[1] 陆一鸣[1] 马兰青[1] 李彦舫[1] 

机构地区：[1]中国人民解放军军需大学植物基因工程研究中心,吉林长春130062

出　　处：《中草药》2004年第11期1286-1289,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的　用 RAPD技术对红景天属 (Rhodiola L.)的大花红景天 R.crenulata、长鞭红景天 R.fastigiata、狭叶红景天 R. kirilowii和高山红景天 R. sachalinensis 4种药用植物进行分子水平鉴定。方法　 CATB法提取红景天属原植物总 DNA,以 2 0 0条随机引物进行随机扩增。结果　用 OPA4 (AATCGGGCTG)、OPB7(GGTGACGCAG)、OPB18(CCACAGCAGT)、OPX14 (ACAGGTGCTG)和 OPZ13(GACTAAGCCC)作随机扩增引物进行随机扩增时 ,可得到识别这些物种基因组 DNA的多态片段。结论　 RAPD法能有效地鉴别红景天类药材 ,把大花红景天、长鞭红景天、狭叶红景天和高山红景天区分开。Object To identify the crude drugs of Rhodiola L., including R. crenulata, R. fastigiata R. kirilowii, and R. saccharinensis by RAPD. Methods General DNA was isolated from fresh leaves of the four species in Rhodiola L. by CTAB and the random amplified polymorphic DNA (RAPD) was used to identify them. Results The polymorphic fragment of DNA fingerprints of different species was compared by primers OPA4(AATCGGGCTG), OPB7(GGTGACGCAG), OPB18, OPX14(ACAGG-TGCTG), and OPZ13(GACTAAGCCC). The studies showed that they have different relationships in heredity and the method of RAPD can be used to identify them easily. Conclusion The raw medicinal matericals Rhodiola L. can be identified by RAPD, and the above-mentioned four species are distingushed.

关 键 词：红景天属 狭叶红景天 大花红景天 法能 原植物 鉴别 高山红景天 RAPD分析 种植 随机扩增 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]