反相高效液相色谱法测定叶酸及其相关物质  

Separation and determination of folic acid and related compounds with high performance liquid chromatography

作　　者：刘树彬[1] 杨更亮[1] 尹俊发[1] 王利娟[1] 李志伟[1] 刘海燕[1] 陈义[2] 

机构地区：[1]河北大学药学院,保定071002 [2]中国科学院化学所分子科学研究中心,北京100080

出　　处：《分析试验室》2004年第11期33-35,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20375010);河北省自然科学基金(202096);科技部基金(No.2002CCA3100;KJCX2 H4);教育部优秀青年教师资助计划;科学院"百人计划"项目

摘　　要：建立了反相高效液相色谱法对叶酸及其相关物质的分离测定方法。对叶酸原料药进行了测定,色谱柱为JASCO C18柱(250mm×4.6mmi.d.,5μm),流动相为V(0.05mol LKH2PO4):V(0.1mol LKOH)∶V(甲醇)=78∶2∶20,流速为1.0mL min,柱温为30℃。叶酸检出限为1.0ng,叶酸的质量分数为98.52%～99 87%;加标回收率为95.22%～100.83%。该法快速简捷,精密度高,重现性好,可用于叶酸的质量控制。A method for the determination of folic acid and related compounds with high performance liquid chromatography has been established. Folic acid and its related compounds were successfully separated on a JASCO C_(18) column (250×4.6 mm i.d.,5 μm) within 12 min by using methanol-phosphoric buffer as mobile phase. The detection limit of folic acid was 1.0 ng. The results of the experiments showed that this method was accurate,rapid, simple and reproducible,and useful for the quality control of folic acid.

关 键 词：反相高效液相色谱法 叶酸 N-对氨基苯甲酰谷氨酸 2 4 5-三氨基-6-羟基嘧啶硫酸盐 

分 类 号：O657.7[理学—分析化学]