利用微束激光将外源基因导入小麦幼胚并获得转基因植株的研究  

STUDY ON INTRODUCTION OF EXOGENOUS GENES INTO THE WHEAT EMBRYO BY USING LASER MICROBEAM AND ACOUISITION OF THE TRANSONIC PLANT

作　　者：王兰岚[1] 宋桂英[1] 张健[1] 付荣昭[1] 马江生[1] 徐正平[1] 舒群芳[1] 孙勇如[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《激光生物学》1993年第2期281-281,278,共2页Acta Laser Biology Sinica

摘　　要：近几年来,国内外学者在小麦遗传转化方面进行了大量研究。在我们实验室利用微束激光成功地将外源基因导入了小麦幼胚细胞并获得转基因植株。 选用小麦京花1号幼胚,PJIT_(101)质粒为实验材料,激光微束仪是中国科学院遗传研究所和重庆京渝激光生物所共同研制的Nd—YAG激光显微照射系统。 将京花1号开花后12—14天幼嫩种子,用0.1％的HgC_(12)灭菌10分钟用无菌水冲洗3—4次,剥取幼胚,用高渗缓冲液泡2—4小时。激光照射前洗去高渗液,加新鲜的Ms培养液,吸取0.5ml含幼胚细胞的悬浮培养液,加40—50ulPJIT_(101)质粒(50ug/ml),注入Rose小室,然后激光照射。This paper deals with a technique of direct introduction of plasmids NPT I gene into wheat emdryo cells by using iaser microbeam. Before irrabiation of laser microdeam, the acceptor cells should ,be treated with high osmotic buffer this ,the introducing frequency is effectively increased and the transgenic plant is obtained ,as well as the stable expression of exogenous genes is realized.

关 键 词：激光 外源基因 小麦 转基因植株 

分 类 号：Q631[生物学—生物物理学]