铜绿假单胞菌消毒剂耐药基因检测  

Detection of disinfectans-resistance gene in Pseudomonas aerugi nosa

作　　者：李智山[1] 邓三季[1] 杨燕[1] 赵建忠[1] 

机构地区：[1]华中科技大学同济医学院附属襄樊医院,襄樊市中心医院,湖北襄樊44102

出　　处：《现代实用医学》2004年第9期530-531,共2页Modern Practical Medicine

摘　　要：目的　调查铜绿假单胞菌 (Pseudomonasaeruginosa ,Pa)的耐消毒剂相关基因存在状况。　方法　用聚合酶链反应 (PCR)法对分离自湖北省襄樊地区住院病人的 35株Pa菌对胺类 (如新洁尔灭 )、胍类 (如洗必泰 )耐药相关基因———qacE基因进行检测。　结果　 35株Pa菌中 19株qacE基因阳性 ,Pa菌qacE基因携带率为 5 4 .3%。　结论　多数所分离的Pa菌已携带耐消毒剂基因。Objective To investigate the condition of disi nf ectans-resistance gene(qacE) in Pseudomonas aeruginosa(Pa). Methods The qacE gene in 35 strains of Pa isolated from Xiangfa n area of Hubei province was detected by polymerase chain reaction(PCR) method. Results Nineteen of 35 Pa strains was qacE positive,and the carrying rate of qacE gene in Pa was 54.3%. Conclusion A maj ority of Pa isolated has carried disinfectans-resistance gene.

关 键 词：假单胞菌 铜绿 消毒药(剂) 基因 

分 类 号：Q786[生物学—分子生物学] R378.991[医药卫生—病原生物学]