2,4-二氯苯基荧光酮分光光度法测定微量锌  

Spectrophotometric Determination of Trace Zinc with 2,4-Dichlorofluorone (DCl-PF)

作　　者：钟志梅[1] 姚俊学[1] 

机构地区：[1]内蒙古大学化学化工学院,呼和浩特010021

出　　处：《内蒙古大学学报（自然科学版）》2004年第6期707-709,共3页Journal of Inner Mongolia University：Natural Science Edition

基　　金：内蒙古自然科学基金(200208020114)

摘　　要：研究了2,4-二氯苯基荧光酮(DCl-PF)与锌的反应条件及应用.实验表明,在pH为10.0的硼砂-NaOH缓冲溶液中,非离子表面活性剂吐温-20(Tween-20)存在下,Zn2+与DCl-PF生成稳定的组成比1∶4的红色配合物,配合物至少稳定2h,最大吸收波长在570nm处,表观摩尔吸光系数为7.6×104L·mol-1·cm-1.Zn2+质量浓度在0～0.64μg/mL范围符合比尔定律,可不经分离直接测定药物、食盐中的微量锌,方法回收率达95%～102%.结果与原子吸收法进行比较,取得较一致的结果.The analytical condition and application of DCl-PF and Zn(Ⅱ) have been studied.The experiment shows that with the presence of surface active agent Tween-20 the color reagent reacts with Zn(Ⅱ) and forms a stable 1∶4 red complex in the medium of Sodium Tetraborate -NaOH buffer solution (pH=10.0).It remains stable for 2 hours at least.The maximum absorption wavelength is at 570 nm and the apparent molar absorptivity is 7.6×10~4L·mol^(-1)·cm^(-1).Beer′s law is obeyed in the concentration range of 0～0.64 μg/mL for Zn(Ⅱ). It has been applied to the determination of trace amounts of Zn(Ⅱ) in medicine and salt without separating it. The recovery rate was 95 to 102 percent. The results were in good agreement with those obtained by atomic absorption spectrometry method.

关 键 词：分光光度法 2 4-二氯苯基荧光酮 锌 药物 

分 类 号：O657.32[理学—分析化学]