L-精氨酸对兔缺血-再灌注心肌线粒体功能及结构的影响  

Effect of L-arginine on function and structure of mitochondria in ischemia-reperfusion myocardial cells in rabbits

作　　者：王万铁[1] 许涛[2] 徐正衸[1] 金可可[1] 潘雪蓉[1] 李东[3] 方周奚[4] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325027 [2]安徽中医学院病理教研室,安徽合肥230038 [3]温州医学院检验系分析中心,浙江温州325027 [4]温州医学院电镜室,浙江温州325027

出　　处：《中国病理生理杂志》2004年第11期2090-2094,共5页Chinese Journal of Pathophysiology

基　　金：浙江省跨世纪学术和技术带头人培养基金(No.992 0 86 ) ;温州市"5 5 1人才工程"培养基金 (No .98113) ;温州市科技局科研基金 (No .S2 0 0 0A2 4 ) ;浙江省卫生厅优秀青年科研基金 (No .2 0 0 1QN0 13)

摘　　要：目的 :探讨L -精氨酸对心肌缺血 -再灌注 (MIR)时心肌细胞线粒体功能及结构的影响。方法 :实验兔 30只 ,随机分为正常对照组 (control组 )、心肌缺血 -再灌注组 (MIR组 )和心肌缺血 -再灌注 +L -精氨酸治疗组 (MRI+L -Arg组 ) ;分别观察心肌线粒体呼吸功能、Ca2 + 浓度 ([Ca2 + ]m)、丙二醛 (MDA)浓度、超氧化物歧化酶(SOD)活性及超微结构的改变和心肌组织三磷酸腺苷 (ATP)、二磷酸腺苷 (ADP)、一磷酸腺苷 (AMP)含量、总腺苷酸量(TAN)、能荷 (EC)的变化。结果 :MIR +L -Arg组线粒体呼吸控制率 (RCR)、Ⅲ态呼吸速率 (V3 )、SOD、面密度 (Sv)、比表面 (δ)明显高于MIR组 ,Ⅳ态呼吸速率 (V4)、[Ca2 + ]m、MDA、体密度 (Vv)、横径 (Hd)显著低于MIR组 ,心肌组织ATP、ADP、TAN及EC均明显高于MIR组 ;且与control组比较 ,V3 、V4、SOD、MDA、Vv、Sv、δ、数密度 (Nv)、纵径 (Vd)及AMP、TAN无明显差异。结论 :L -精氨酸可通过降低氧自由基水平和减轻钙超载 ,而改善缺血AIM: To study the effect of L-arginine (L-Arg) on function and structure of mitochondria in ischemia-reperfusion (MRI) myocardial cells. METHODS: Thirty rabbits were randomly divided into three groups (n=10 in each), control group, MIR group and MIR+L-Arg group. The mitochondrial respiratory function, Ca^(2+) concentration ([Ca^(2+)]m), malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. Meanwhile, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), total adenylic acid number (TAN) and energy charge (EC) in the myocardial tissue were respectively measured. Moreover, the ultrastructure changes in myocardial mitochondria were observed during MIR. RESULTS: The mitochondrial respiratory control rate (RCR), velocity 3 (V_3), SOD, surface density (Sv) and specific surface (δ) in MIR+L-Arg group were higher than those in MIR group, velocity 4 (V_4), [Ca^(2+)]m, MDA, volume density (Vv), horizental diameter (Hd) were lower than those in MIR group. ATP, ADP, TAN and EC levels of myocardial tissue were higher than those in MIR group. There was no significant difference between MIR+L-Arg and control group in V_3, V_4, SOD, MDA, Vv, Sv, δ, Nv, Vd, AMP and TNA. CONCLUSION: It is suggested that L-Arg improves the function and structure of mitochondria in myocardial cells in the reperfusion injury after myocardial ischemia by decreasing oxygen free radical level and Ca^(2+) overload in the mitochondria. [

关 键 词：心肌缺血 线粒体 心脏 精氨酸 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学]