肥胖症患者瘦素受体基因外显子4变异特征  

Characteristics of exon 4 variation in leptin receptor gene of patients with obesity

作　　者：侯英[1] 丁久立[1] 段滨红[1] 冯琨[1] 王亦薇[1] 李贤厚[1] 朱巍[1] 杨玉芝[1] 

机构地区：[1]黑龙江省医院内分泌代谢科,黑龙江省哈尔滨市150036

出　　处：《中国临床康复》2004年第33期7422-7423,共2页Chinese Journal of Clinical Rehabilitation

基　　金：黑龙江省自然科学基金资助项目(D00-41)~~

摘　　要：目的:探讨瘦素受体基因外显子4变异情况及是否与肥胖症相关。方法:选择肥胖症患者犤体质量指数(BMI)≥28kg/m2犦和正常人(18.5≤BMI<23kg/m2)各50例,运用PCR-SSCP方法,检测瘦素受体基因外显子4的变异情况。结果:50例肥胖症患者瘦素受体基因外显子4未发现碱基改变。结论:瘦素受体基因外显子4中519位点变异不是肥胖症的遗传因素。AIM:To investigate the possible relationship between variant of exon 4 in leptin receptor (LEPR) gene and obesity.METHODS: Genomic DNA was extracted from 50 obese subjects [body mass index (BMI) ≥28 kg/m2] and 50 normal individuals(18.5≤BMI< 23 kg/m2), and the variation of exon 4 was determined by PCR-SSCP method.RESULTS: There was no abnormal change in 50 obese cases during detection.CONCLUSION: Variation of exon 4 in LEPR gene at site 519 is not a genetic factor of obesity.

关 键 词：变异(遗传学) 蛋白质类/遗传学 多态性 限制性片段长度 肥胖症/遗传学 聚合酶链反应 瘦素 

分 类 号：R589.2[医药卫生—内分泌]