松科10属植物叶子内皮层具有凯氏带的新证据  

Occurrence of Casparian strips in the leaf endodermis of 10 genera of pinaceae

作　　者：唐熙[1] 吴小琴[1] 吕洪飞[1] 林金星[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《电子显微学报》2004年第5期560-565,共6页Journal of Chinese Electron Microscopy Society

基　　金：中国科学院创新重点项目基金资助项目(批准号:KSCXZ SW 108).

摘　　要：本文应用荧光显微技术,结合检测凯氏带的特异染料小檗碱和甲苯胺蓝对染方法,对松科10属13种植物叶子的冰冻切片进行了观察,确认在松科的10属植物叶子中均有内皮层凯氏带加厚,纠正了前人关于在松科植物某些属的叶子中不存在凯氏带的结论。根据内皮层细胞排列模式和凯氏带加厚程度,我们将松科的10属分成两大类。通过酶解分离我们得到典型的凯氏带网状结构,说明其能够抵御酶解,其原因可能是成份含木质素和木栓质。Cross sections of leaves from 13 species belonging to 10 genera of Pinaceae were prepared by cryo-microtome and counter-stained by berberine/aniline blue, then were observed under the fluorescence microscope. It was determined that the endodermis of all genera possessed Casparian thickening, in contrast to some of the earlier conclusions that Casparian strips did not occur in the endodermis of several genera. In terms of arrangement patterns of endodermal cells and thickening mode of Casparian strips, two types could be identified among the leaves of ten genera. In addition, typical net-like structures of Casparian strips were isolated from the leaves after incubating with enzymes, indicating that they could stand enzyme degradation and were mainly composed of lignin and suberin.

关 键 词：凯氏带 内皮层 松科 植物叶子 细胞 荧光 木质素 冰冻切片 小檗碱 观察 

分 类 号：Q944[生物学—植物学]