链格孢菌毒素对紫茎泽兰叶组织超微结构的影响  

Effect of Alternaria alternata toxin on ultrastructure of leaf tissue of Eupatorium adenophorum sprengel

作　　者：常缨[1] 王学东[2] 强胜[1] 

机构地区：[1]南京农业大学生命科学学院杂草研究室,江苏南京210095 [2]东北农业大学生命中心,黑龙江哈尔滨150030

出　　处：《电子显微学报》2004年第5期566-570,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家863项目(No.2001AA246012);国家自然科学基金资助项目(No.30170619);国家基础研究专项(973项目)(No.2002CB111402);江苏省自然科学基金资助项目(No.BK2001066);江苏省博士后基金8590课题资助项目.

摘　　要：用导致世界恶性杂草紫茎泽兰自然致病的链格孢菌(Alternariaalternata(Fr.)Keissler)强致病菌株所产生的毒素处理紫茎泽兰顶端第2对真叶,研究该毒素对紫茎泽兰叶肉细胞超微结构的影响。结果表明毒素处理4h后,细胞出现质壁分离,叶绿体变形;24h后叶肉细胞的细胞壁变形甚至断裂,质膜断裂;叶绿体变形严重,类囊体片层解体,叶绿体膜膨胀、断裂。叶肉细胞的叶绿体受损伤程度大,暗示了毒素的作用方式很可能是抑制光合作用。The upper second leaves were treated with the toxin extracted from the isolate of Alternaria alternata(Fr.)Keissler,which caused serious leaf-spot disease of Eupatorium adenophorum,in order to observe the effect of the toxin on the ultrastructure of leaf tissue.The results showed that plasmolysis and chloroplast deformation only took place after treatment for 4h.24h after,the ultrastructure of leaf tissue was badly damaged,including the deformed and broken cell wall,the broken plasmalemma,the misshaped chloroplast,decomposed chloroplast lamellae and broken envolopen of chloroplast.These results suggested that the possible mode of the toxin is inhibiting photosynthesis.

关 键 词：紫茎泽兰 兰叶 链格孢菌 叶肉细胞 毒素 叶绿体 恶性杂草 超微结构 后叶 严重 

分 类 号：S451[农业科学—植物保护]