沐舒坦对盐酸吸入后大鼠氧化抗氧化系统的影响  

Oxidative and anti-oxidative effects of ambroxol on acute hydrochloric acid-induced lung injury in rats

作　　者：赵双平[1] 郭曲练[1] 王瑞珂[1] 王锷[1] 

机构地区：[1]中南大学湘雅医院ICU,长沙410008

出　　处：《中南大学学报（医学版）》2004年第5期586-588,共3页Journal of Central South University ：Medical Science

摘　　要：目的 :观察沐舒坦对盐酸吸入性肺损伤是否具有保护作用 ,并探讨其可能机制。方法 :30只健康SD大鼠随机分成 3组 (A组 :生理盐水吸入组 ;B组 :稀盐酸吸入组 ;C组 :稀盐酸吸入 +沐舒坦处理组 )。C组予以 5 0mg/kg沐舒坦腹腔注射 ,每天 1次 ,连续 3d ,A ,B组则以等体积生理盐水腹腔注射代替。第 3d腹腔注射沐舒坦或生理盐水 30min后 ,A组以 1.2ml/kg生理盐水经气管注入作为对照组 ;B ,C组用 pH 1.2 5盐酸 +生理盐水混合液1.2ml/kg气管注入。观察盐酸注入 5h后大鼠动脉血气、肺组织匀浆和血清中SOD活力、MDA含量。结果 :沐舒坦预先处理能抑制盐酸吸入后引起的PaO2 下降 ,3组间比较差异有统计学意义 (P <0 .0 1)。盐酸吸入 5h后B组肺匀浆和血中SOD活力均明显下降、肺匀浆MDA升高 ,而C组在盐酸吸入 5h后肺匀浆以及血中SOD活力明显高于B组 ,肺匀浆MDA则比B组明显低 (P <0 .0 1) ,但 3组血中MDA值比较差异无统计学意义 (P >0 .0 5 )。结论 :沐舒坦对盐酸吸入性肺损伤有保护作用 ,抑制脂质过氧化反应。Objective To evaluate the protective effect of ambroxol on acute hydrochloric acid- induced lung injury in rats. Methods Thirty pathogen-free SD rats were randomly divided into 3 groups: Group A (n=10) served as control group, and received intracheal instillation of normal saline (NS, pH5.3,1.2 ml/kg) with pre-treatment of intraperitoneal NS;Group B (n=10)received intracheal instillation of hydrochloric acid /NS (pH1.25,1.2 ml/kg) with pre-treatment of intraperitoneal NS; and Group C received intracheal instillation of hydrochloric acid /NS (pH1.25,1.2 ml/kg) with pre-treatment of intraperitoneal ambroxol (50 mg/kg/d, 3 days). Five hours after the instillation of the injury vehicle, the arterial gas was examined, the levels of superoxide dismutase (SOD), malondialdehyde(MDA) in the blood and homogenate of harvested lung were assayed respectively. Results PaO 2 in Group B was significantly lower than that in Group A and C (P<0.01),and pH and PaCO 2 in the 3 groups had little difference (P>0.05).MDA in the lungs of Group B increased obviously,and levels of SOD in the lung and blood decreased significantly in Group B (Group B vs Group A,P<0.01).Levels of SOD of Group C in the lung and blood were markedly higher than those of Group B (P<0.01),MDA of Group C was obviously lower than that of Group B (P<0.01),and MDA of blood in the 3 groups had no significant difference (P>0.05).Conclusion Ambroxol can inhibit lipid peroxidation and increase antioxidant activity, which may be one of the mechanisms in protecting lung tissue from hydrochloric acid-induced injury.

关 键 词：沐舒坦 吸入性肺损伤 生理盐水 肺匀浆 腹腔注射 SD大鼠 SOD活力 抗氧化系统 MDA 抗氧化能力 

分 类 号：R965[医药卫生—药理学]