水分胁迫下水稻剑叶中多胺含量的变化及其与抗旱性的关系  

Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought resistance of Rice Cultivars under Water Deficit Stress

作　　者：杨建昌[1] 张亚洁[1] 张建华[2] 王志琴[1] 朱庆森[1] 

机构地区：[1]扬州大学农学院,江苏扬州225009 [2]香港浸会大学生物系

出　　处：《作物学报》2004年第11期1069-1075,共7页Acta Agronomica Sinica

基　　金：国家重点基础研究发展规划项目 (G1 9990 1 1 70 4 )。

摘　　要：以 6个中熟水、陆稻品种为材料 ,研究了在不同土壤水分状况下剑叶中腐胺 (Put)、亚精胺 (Spd)和精胺 (Spm)含量的变化及其与抗旱性的关系。结果表明 ,供水充足 (对照 )的叶片中Put、Spd和Spm含量随叶片的衰老而缓慢下降 ,与品种的抗旱性没有明显联系。水分胁迫处理 (土壤水势保持 -0 0 5MPa)后 ,叶片中Put、Spd和Spm含量均显著增加 ,增加的量以Put >Spd >Spm ;抗旱品种叶片中多胺累积开始较早、持续时间长 ,Spd和Spm的相对含量 (胁迫处理 对照 )较高 ,干旱敏感性品种则相反。Put和Spd或Spm开始在叶片中累积的叶水势阈值 ,抗旱品种分别为 -0 5～ -0 53MPa和-0 61～ -0 62MPa,干旱敏感性品种分别为 -0 72～ -0 74MPa和 -0 81～ -0 83MPa。Spd和Spm的相对含量与品种的抗旱系数呈极显著的正相关 (r =0 92 1 ～ 0 984 ,P <0 0 1 ) ,Put的相对含量与抗旱系数的相关值因测定时期不同而异。在水分胁迫初期分别用 0 1mmol LSpd或Spm喷施叶片 ,籽粒产量较仅用清水处理的对照显著增加 ,用 0 2mmol LPut处理后的产量与对照差异不显著 ,用 1mmol LMGBG [甲基己二醛 双 (脒基腙 ) ,多胺合成抑制剂 ]处理 ,产量较对照显著降低。上述结果说明 。With six mid maturing lowland and upland rice cultivars as materials, the changes in contents of putrescine (Put),spermidine (Spd) and spermine (Spm) in the flag leaf and their relationship with drought resistance under different status of soil moisture were investigated. The contents of Put, Spd and Spm in the leaf were decreased with the senescence of the leaf and were not correlated with the drought resistance of the cultivar under the well watered (control) condition. They were significantly increased under the water deficit stress (WS, soil water potential was kept at -0.05 MPa), and the increment trend was Put>Spd>Spm. The polyamines in the leaf were accumulated earlier and for a linger time under WS, and the relative contents (WS/control) of Spd and Spm were greater for drought resistant cultivars than for drought sensitive ones. The leaf water potential thresholds at the beginning of the accumulation of Put and Spd or Spm were (-0 50)-(-0 53) MPa and (-0 61)-(-0 62) MPa respectively for drought resistant cultivars and (-0 72)-(-0 74) MPa and (-0 81)-(-0 83) MPa respectively for drought sensitive ones. The relative contents of Spd and Spm were positively and very significantly correlated with drought resistant coefficients of cultivars ( r =0 921 ** -0 984 ** , P <0 01), and the correlation between the relative content of Put and drought resistant coefficients varied with determination stages. The grain yield was significantly increased when leaves were sprayed with 0 1 mmol/L Spd or Spm at the initial of WS. It was not affected by the treatment of 0 2 mmol/L Put but the grain yield was significantly decreased by treated leaves with 1 mmol/L MGBG [methlglyoxal bis (guanyl hydrazone), an inhibitor of polyamine synthesis]. The results suggest that the changes of polyamines in the flag leaf under WS are closely associated with drought resistance of cultivars. Earlier and longer duration of polyamines accumulation in the leaf under WS and greater relative contents of Spd an

关 键 词：水稻 水分胁迫 多胺 腐胺 亚精胺 精胺 抗旱性 

分 类 号：S511[农业科学—作物学]