水稻苯达松敏感致死基因(ben)的电子杂交定位和基因预测  

Location of Bentazon Sensitive Lethality Gene ( ben ) on Rice Chromosome and Its Gene Forecast in silico

作　　者：杨剑波[1] 向太和[1] 李莉[1] 王永杰[1] 黄大年[2] 

机构地区：[1]安徽省农业科学院水稻研究所,安徽合肥230031 [2]中国水稻研究所,浙江杭州310006

出　　处：《作物学报》2004年第11期1152-1158,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划 ( 2 0 0 1AA2 110 6 1);国家攻关计划 ( 2 0 0 1BA30 2B)资助项目

摘　　要：来自水稻突变体的苯达松敏感致死基因 (ben)能够作为一种除草剂筛选标志用于杂交水稻种子的安全生产。本研究利用我们已得到的与Ben ben基因紧密连锁的两个RAPD标记 (OPG1 8 972 ,OPG1 8 94 3 )以及与该标记高度同源的跨叠克隆Contig1 0 96 8(来自中国水稻基因组框架序列 ,全长 82 73bp)的序列信息 ,沿标记的两端设计新的PCR引物 ,以携带苯达松抗性基因 (Ben)和苯达松敏感基因 (ben)的水稻近等基因系为模板 ,通过对扩增产物的序列拼接和比对 ,成功地将OPG1 8 972和OPG1 8 94 3标记从 972bp和 94 3bp步移延伸到 81 3 8bp和 81 0 3bp(提交到GenBank的登记号分别为AY1 81 2 0 4和AY1 81 2 0 3 )。并利用电子杂交将延伸后的序列定位于水稻第 2染色体上 1 5 0 5cM处 ,在对标记两端及其附近的序列进行生物信息学分析后 ,我们初步预测Ben基因可能是编码某种受体蛋白的基因或催化 6 OHA bentazon sensitive lethality gene obtained from rice mutant can be used as a herbicide selection marker to ensure safe production of hybrid rice seeds.In our previous study,two RAPD markers OPG18/972 and OPG18/943 closely linked to Ben/ben gene were anchored on Contig 10968 based upon homology comparison of the sequence.Here a set of new PCR primers were designed in series according to Contig 10986 sequence around the markers.By alignment and comparison of PCR fragments amplified on with the template DNA from Ben/ben near isogenic lines of rice,the markers OPG18/972 and OPG18/943 were successfully walked and elongated from 972 bp and 943 bp to 8 138 bp and 8 103 bp (GenBank accession:AY181204 and AY181203 respectively) which were anchored to chromosome 2 at 150 5 cM in silico .Bioinformatics analysis displayed a clue that Ben gene might code a receptor protein or a certain starch synthase like protein which catalyzed 6 hydroxybentazon (6 OH bentazon) into the glucose conjugate.

关 键 词：水稻 苯达松敏感致死基因 定位 预测 电子杂交 

分 类 号：S511[农业科学—作物学]