卵巢过度刺激综合征动物模型制备  

Establishment of the animal model of ovarian hyperstimulation syndrome

作　　者：孙丽华[1] 王咏梅[1] 史逸超[1] 薛文群[1] 姚兵[1] 

机构地区：[1]无锡市妇幼保健院,214002

出　　处：《江苏医药》2004年第11期820-821,共2页Jiangsu Medical Journal

基　　金：江苏省社会发展基金资助课题 (编号BS2 0 0 2 3 40 )

摘　　要：目的　构建卵巢过度刺激综合征 (ovarianhyperstimulationsyndrome ,OHSS)的大鼠模型。方法　 18只雌性Wistar大鼠 ,2 2日龄 ,体重 4 2 0～ 5 0 0 g ,随机分成超排卵组 1、组 2和对照组。卵巢过度刺激组从 2 2～ 2 5日龄连续 4d皮下注射马促性腺激素 (eCG) 10IU(0 1ml生理盐水稀释 ) ,在第 2 6日龄皮下注射绒毛膜促性腺激素 (hCG) 30IU、10 0IU ;对照组从第 2 2～ 2 6日龄连续 5d注射生理盐水。在hCG注射 4 8h后 (即 2 8日龄 )尾静脉注射 1%EB(Evensblue)染料 0 1ml,6 0min后腹腔灌注 5ml生理盐水 ,回收灌注液并置一个含有 5 0 μl0 1NNaOH的试管中 ,双侧卵巢分别称重后 ,把左侧卵巢置于 4ml甲酰胺中萃取EB。结果　卵巢过度刺激综合征组卵巢重量组 1为 (4 0 0 5 0±5 1 0 6 )mg ;组 2 (4 2 1 83± 88 93)mg ,显著高于对照组的 (32 82± 5 2 0 )mg(P <0 0 1)。卵巢EB浓度组 1为 (73 18± 11 38)ng/mg ;组 2 (73 2 6± 18 4 4 )ng/mg ,显著高于对照组 (35 87± 5 14 ) ng/mg(P <0 0 1)。组 1腹腔EB浓度 (13 994± 0 4 5 6 ) μg/ml,组 2 (4 15 4± 1 0 90 ) μg/ml,显著高于对照组 (0 333± 0 0 82 ) μg/ml(P <0 0 1)。 结论　利用适当日龄Wister大鼠构建OHSS动物模型成功。Objective To establish the rat model of ovarian hyperstimulation syndrome(OHSS).Methods 18 female Wistar rats aged 22 days and weighted 42.0-50.0 g were divided randomly into three groups:group-1(OHSS-1),group-2(OHSS-2)and control group.The rats in group 1 and group 2 were injected s.c. with 10 IU of equine chorionic gonadotrophin(eCG) in 0.1 ml 0.9% saline for 4 consecutive days,and 30 IU,100 IU of human chorionic gonadotrophin(hCG)in 0.1 ml of 0.9% saline on the 5th day (the 26th day of life).Meanwhile the rats in control group were given 0.1 ml 0.9% saline instead of eCG/hCG for 5 consecutive days.Under inhalation anaesthesia,rats received 0.1 ml of 1% Evans blue dye(EB)solution via the tail vein.The peritoneal cavity was irrigated with 5 ml of 0.9% saline for 60 min after the EB injection. The irrigated fluid containing 0.05 ml of 0.1 N NaOH was centrifuged at 900 g for 10 min at room temperature.The EB concentrations of the irrigated fluids were detected at the wavelength of 600 nm with a spectrophotometer.The bilateral ovaries were weighted.Then the left ovary was incubated in 4 ml of 65℃ formamide for 24 h.Results Ovary weight,ovarian EB content peritoneal EB concentration of ovarian hyperstimulation groups were significantly higher than those of control group(P<0.01).Conclusion The animal model of OHSS were secured by injected eCG and HCG.The model may be useful for research on the pathogenesis of the ovarian hyperstimulation syndrome.

关 键 词：对照组 卵巢过度刺激综合征 生理盐水 动物模型 OHSS 皮下注射 CG 水稀释 EB 制备 

分 类 号：R711.75[医药卫生—妇产科学] R737.31[医药卫生—临床医学]