SARS患者特异性抗体水平的动态监测  

Antibodies in Patients with Severe Acute Respiratory Syndrome: Dynamic Observation

作　　者：韩晓芳[1] 宋壮志[2] 李慧君[1] 王文灏[1] 吴翠平[2] 杜英[1] 梁建林[1] 

机构地区：[1]内蒙古自治区医院,内蒙古呼和浩特010017 [2]内蒙古自治区疾病预防控制中心,内蒙古呼和浩特010000

出　　处：《中华医院感染学杂志》2004年第11期1239-1241,共3页Chinese Journal of Nosocomiology

基　　金：内蒙古自治区医教卫生科技攻关项目

摘　　要：目的　探讨 SARS患者不同时期体内特异性抗体水平的变化。方法　采用间接 EL ISA法检测 34例早期、恢复期 SARS患者 ,39例治愈后随访者 ,2 0 1例一线医护人员及 30例健康体检者血清中 SARS病毒 Ig G和 Ig M抗体 ,同时对 18例 SARS患者及 4 0例医护人员的泪液也做了检测。结果　 Ig M抗体的阳性率在疾病早期和恢复期差异无显著性 ( P>0 .0 5 ) ,而 Ig G抗体的阳性率在恢复期高于早期差异具有非常显著性 ( P<0 .0 0 5 ) ;12例系列血清 Ig G抗体含量在恢复组和随访组差异无显著性 ( P>0 .0 5 ) ,而 Ig M抗体含量在随访组大大降低 ,差异有显著性 ( P<0 .0 5 ) ;一线医护人员 Ig G抗体的阳性率为 1.99% ,健康体检者 Ig G和 Ig M抗体均为阴性 ;SARS早期、恢复期患者及一线医护人员的泪液中均未检出 SARS病毒抗体。结论　 SARS病毒 Ig M抗体在发病 3个月后基本阴转 ,而 Ig G抗体仍持续高水平阳性 ,有可能成为保护性抗体 ;OBJECTIVE To investigate the change in specific antibodies levels in patients with severe acute respiratory syndrome (SARS) during different stages. METHODS SARS-Cov IgG and IgM were detected by indirect ELISA in 34 patients with SARS at the early and recovery stages and 39 follow-up cases, 201 front-line doctors and nurses, and 30 healthy volunteers and the tears of some above cases were detected too. RESULTS The positive ratio of IgM in patients with SARS during the early and recovery stages was not significant (P>0.05), while that of IgG at the recovery stage was higher than that at the early one, which was very significantly different (P<0.005). The contents of IgG in serial serum of 12 cases at the recovery and follow-up stages were not significant (P>0.05). While the content of IgM at the follow-up stage was reduced a lot, which was significantly different (P<0.05). The positive ratio of IgG tested among the front-line doctors and nurses was 1.99%, IgG and IgM were not found in the tears of patients with SARS and the front-line doctors and nurses. CONCLUSIONS Anti-SARS IgM might disappear gradually 3 months after the patient infected with SARS-Cov, while IgG still remains high, which will be likely to be protective antibodies. The dynamic observation of the specific antibodies in patient with SARS can be applied in the study for epidemiology and serodiagnosis.

关 键 词：SARS病毒 特异性抗体 血清 泪液 

分 类 号：R183.3[医药卫生—流行病学]