DDVP对德国小蠊乙酰胆碱酯酶和谷胱甘肽S-转移酶活性的影响  

Effects of DDVP at differenct concentrations on acetycholine esterase and glutathione s-transferase activity of German cockroaches (Blattella germanica

作　　者：郭丽[1] 沈孝兵[1] 

机构地区：[1]东南大学丁家桥校区公共卫生学院,南京210009

出　　处：《医学动物防制》2004年第10期600-602,共3页Journal of Medical Pest Control

基　　金：铁道部站车卫生科研基金资助项目(J99Z217)

摘　　要：目的:了解不同浓度敌敌畏(DDVP)对德国小蠊乙酰胆碱酯酶和谷胱甘肽S-转移酶活性的影响,为探讨德国小蠊抗性机制及建立抗性生化检测方法提供理论依据。方法:采用分光光度法和微量板酶动力学法分别测定不同浓度DDVP作用下德国小蠊乙酰胆碱酯酶和谷胱甘肽S-转移酶的活性,应用SAS软件进行统计分析。结果:随着施药浓度增加,乙酰胆碱酯酶和谷胱甘肽S-转移酶活性逐渐降低,施药浓度在0.01％及以上各组的乙酰胆碱酯酶酶活性与对照组有显著性差异:施药浓度在0.02％及以上各组的谷胱甘肽S-转移酶活性与对照组有显著性差异。结论:乙酰胆碱酯酶是DDVP的主要靶标酶之一。谷胱甘肽S-转移酶是DDVP的重要代谢酶。Objective: To measure the effect of differenct concentrations DDVP to the acetycho-line esterase and glutathione s-transferase activity of German cockroaches (Blattella germanica). Methods: measured by visible spectrophotometry and kinetic microplate. analysized by SAS soft. Result: the result showed that AChE and GST activity of Blattella germanica decreased correlated with the concentration of DDVP increasing and the AChE and GST activity of treated group was significantly lower than the contrast group. Conclusion: acetycholine esterase was the main target enzyme of DDVP and glutathione s-transferase was the important metabolic enzyme.

关 键 词：谷胱甘肽S-转移酶 乙酰胆碱酯酶 德国小蠊 DDVP 对照组 酶动力学法 影响 酶活性 施药浓度 抗性机制 

分 类 号：R979.8[医药卫生—药品] R184.39[医药卫生—药学]