不同构成人参皂苷制备的理论模拟与实验研究  

Theoretical simulation and experimental research on constructing different compositions of ginsenoside

作　　者：倪力军[1] 王君华[1] 史晓浩 王宁 

机构地区：[1]华东理工大学化学与制药学院,上海200237 [2]上海雷允上药业有限公司,200002

出　　处：《中成药》2004年第11期867-870,共4页Chinese Traditional Patent Medicine

基　　金：国家科技部十五攻关项目 (2 0 0 1BA70 1A0 4) ;国家中医药管理局中医药科学技术研究专项 (0 2- 0 3ZP3 0 )

摘　　要：目的 :构造成份比例不尽相同的人参皂苷提取物 ,为药理药效实验提供多种类型的试样。方法 :采用UNIFAC基团贡献法进行液液萃取模拟 ,选择合适的萃取剂体系进行实验 ,并通过HPLC检测不同萃取剂体系下的人参皂苷。结果 :随着萃取剂体系的变化 ,几种主要皂苷的含量发生相对变化 ,可构造出一系列具明显特征的人参皂苷提取物 ;在含量较高的几种皂苷中 , Rb1 较易和 Re分离 ,而与 Rd较难分离 ,实验与理论预测的结果一致。结论 :理论计算结合萃取剂体系的优化可构造有明显差异的人参皂苷提取物。AIM: To Structure a series of diversifying combination of ginsenoside in order to provide the different samples for the pharmacological and pharmacodynamics experiments. METHODS: UNIFAC group contribution method is used for simulating the liquid liquid extraction, the proper extractants are chosen to carry out the experiments, and then the contents of the ginsenosides obtained with different extractants are detected through HPLC. RESULTS: The contents of the main ginsenoside varied with the change of the extractants' ratio, as a result a series of diversifying combination of ginsenoside are constructured. Within the ginsenoside which has greater amount, ginsenoside Rb 1 can be easily separated from Re, while Rd is hard to be separated, the experimental data were accordant to the theoretical prediction. CONCLUSION: Theoretical calculation combined with the optimizing of the extractants can structure a series of distinguished ginsenoside.

关 键 词：人参皂苷 液液萃取 模拟 

分 类 号：R284.2[医药卫生—中药学]