异鼠李素诱导A549细胞凋亡的研究  

Study on apoptosis of human A549 cells induced by isorhamnetin

作　　者：朱玲[1] 周黎明[1] 杨春蕾[2] 肖静[1] 王正荣[1] 王浴生[1] 

机构地区：[1]四川大学基础与法医学院,成都610041 [2]四川大学细胞生物学教研室,成都610041

出　　处：《中国抗生素杂志》2004年第11期687-690,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金资助(编号39970275;30070278)。

摘　　要：目的观察异鼠李素是否能诱导人肺腺癌细胞株A549细胞凋亡及相关基因的变化。方法20μg/ml异鼠李素处理A549细胞,光镜电镜下观察细胞形态;进行集落形成实验,MTT法测细胞生长抑制率;流式细胞仪检测凋亡峰及bax,bcl-2等凋亡相关基因的表达。结果10～640μg/ml异鼠李素可抑制A549细胞生长,抑制率有剂量依赖性。流式细胞仪检测20μg/ml异鼠李素处理后出现明显凋亡峰;药物可使bax表达上调,bcl-2表达明显下降,表达改变有浓度依赖性。结论异鼠李素可抑制A549细胞生长,诱导其凋亡,其诱导凋亡的作用与凋亡相关基因抗凋亡基因bcl-2表达明显下调,bax表达上调有关。Objective To observe the apoptosis of human cell line A549 induced by isorhamnetin and to investigate the change of apoptosis-associated genes and DNA breakdown. Methods A549 cells were treated with 10～320μg/ml isorhamnetin and observed and analyzed under the light-microscope and electronic ~microscopy ; the growth inhibition was analyzed by MTT; the apoptosis and the expression-associated genes peaks were detected with flow cytometry (FCM). Results Isorhamnetin remarkably inhibited the growth of A549 cells, showing the apoptotic changes in microscope. 20μg/ml isorhamnetin could induce A549 cells apoptosis and up-regulate the expression of apoptosis gene bax and down-regulate the expression of anti-apoptotic gene bcl-2. Conclusion Isorhamnetin can induce apoptosis of A549 cell and apoptosis is related to the down-regulation of anti-apoptotic genes and up-regulation of apoptotic genes.

关 键 词：异鼠李素 A549细胞 凋亡 

分 类 号：R965[医药卫生—药理学]