冠状动脉支架术后C反应蛋白升高与树突状细胞的关系  

Elevation of blood C-reactive protein after coronary stent implantation is related to the activation of dendritic cells

作　　者：李大主[1] Sharma Ranjit 曾秋棠[1] 田园[2] 冯义柏[1] 王祥[1] 曹林生[1] 郭和平[1] 王敏[1] 

机构地区：[1]华中科技大学协和医院心内科华中科技大学同济医学院心血管研究所,武汉430022 [2]华中科技大学协和医院外科免疫室

出　　处：《临床心血管病杂志》2004年第11期641-643,共3页Journal of Clinical Cardiology

摘　　要：目的 :研究冠状动脉支架术后高敏C反应蛋白 (hsCRP)的升高与树突状细胞 (DC)的关系。方法 :术前及术后 2d分离 11例冠状动脉支架术患者外周血单个核细胞 (PBMC) ,在含粒细胞巨噬细胞集落刺激因子(GM CSF)和白细胞介素 4 (IL 4 )的培养条件下制备DC。用流式细胞仪检测DC表面共刺激分子CD86 (B7 2 )的表达 ;混合淋巴细胞反应 (MLR)检测DC对同种异体T淋巴细胞的刺激能力 ;ELISA法测定MLR上清液中的细胞因子 ;比浊法测定hsCRP浓度 ,并探讨CD86表达与hsCRP的相关性。结果 :与术前比较 ,术后 2dDC表面CD86的表达明显增高 ;对T淋巴细胞刺激的能力增强 ;经DC刺激的淋巴细胞分泌促炎细胞因子 (IL 1β、IL 6、TNF α)增多 ,抑炎细胞因子 (IL 10 )减少 ;同时血hsCRP升高 ,且CD86的表达与血hsCRP水平正相关。结论 :支架术后DC的功能被激活 。Objective:To investigate the relation between elevation of blood high sensitive C reactive protein (hsCRP) and function of dendritic cells (DC) after coronary stent implantation. Method:PBMC from 11 patients before and 2 days after coronary stenting were incubated and induced to mature DC in the completed medium containing GM-CSF and IL-4.The flow cytometric analysis were used to detect the expression of co-stimulating factor CD86(B7-2)on DC. The stimulating capacity of DC was determined in allogenic mixed lymphocyte reaction (MLR).ELISA was used to analyze the level of cytokines(IL-1β, IL-6, IL-10 and TNF-α) in the medium of MLR. Relationship of expression of CD86 to blood hsCRP was also analyzed. Result:When compared with before stenting, CD86 on DC 2 days after was much more expressed ; the stimulating capacity of DC in MLR was higher;T lymphocytes in MLR secreted higher levels of pro-inflammation cytokines(IL-1β,IL-6 and TNF-α) and lower level of anti-inflammation cytokine(IL-10). At the same time hsCRP was elevated; hsCRP was positively related to the expression of CD86. Conclusion:DCs in coronary stenting are activated, which may be the cause of elevation of blood hsCRP.

关 键 词：冠状动脉狭窄 支架 C反应蛋白 树突状细胞 

分 类 号：R654.3[医药卫生—外科学]