花生幼胚RNA提取方法研究  

Study on the methods of RNA isolation from young peanut embryos

作　　者：张君诚[1] 魏彦飞[2] 唐荣华[2] 官德义[2] 刘思衡[2] 庄伟建[2] 

机构地区：[1]三明高等专科学校化学与生物工程系,福建三明365004 [2]福建农林大学作物学院油料研究所

出　　处：《福建农林大学学报（自然科学版）》2004年第1期1-4,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30070481);福建省教育厅资助项目(JA02256);福建省三明市科技局资助项目(2003-C-3).

摘　　要：花生(ArachishypogaeaL.)幼胚体积很小,不易剥取且富含多糖、酚类等化合物,因此以花生幼胚为材料提取RNA时会遇到很大困难.采用改进的Trizol法和异硫氰酸胍一步法成功提取出了高质量的花生幼胚总RNA,后续实验证明具有良好的逆转录活性.介绍了花生幼胚快速剥取的方法.It was difficult to isolate total RNA from young peanut embryo, which was not only too small to be obtained, but also contained polysacchrides, phenolic compounds, many unidentified secondary metabolites and so on. Two methods of the extraction of RNA from young peanut embryos, improved Trizol method and one step of guanidiumthiocyanate, were introduced and discussed in this paper. The subsequent experiments proved the high quality of the RNA acquired by the two methods. Meanwhile, the method of rapidly sampling young peanut embryos was also presented.

关 键 词：幼胚 花生 RNA提取方法 剥取 总RNA 异硫氰酸胍 逆转录 取出 多糖 酚类 

分 类 号：S565.2[农业科学—作物学] S512.1