农杆菌介导获得转水稻草矮病毒NS3基因水稻植株  

Agrobacterium-transformed rice plants harboring NS3 gene of Rice grassy stunt virus were obtained

作　　者：林丽明[1] 吴祖建[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2004年第1期60-63,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金(39970486);福建省自然科学基金(C0310010);福建省青年科技人才创新基金(2002J028)资助项目.

摘　　要：构建了包含水稻草矮病毒(Ricegrassystuntvirus,RGSV)NS3基因的植物表达载体pCBTNSv3,应用农杆菌介导法,将NS3基因导入水稻愈伤组织中,获得了转RGSVNS3的水稻转基因植株,总DNA经PCR、Southern点杂交鉴定,初步证实NS3基因已整合到水稻基因组中.The plant expression vector pCBTNSv3 containing vRNA3 NS3 gene was obtained by replacing the exterior segment of pCBTNSv6 constructed by doctor Zhang Chunmei of our insititute. The recombinant plasmid of pCBTNSv3 was introduced into Agrobacterium tumefaciens by triparental mating. The positive clones were then used in the transformation of rice. By the Agrobacterium-mediated method, the transgenic rice plants were regenerated. PCR and Southern blotting analysis of the total DNA extracted from transgenic plants showed that the NS3 gene of RGSV had been integrated into the rice genome.

关 键 词：水稻 草矮病毒 转基因植株 农杆菌介导法 稻草 愈伤组织 总DNA NS3基因 PCR 鉴定 

分 类 号：S511[农业科学—作物学] R373.21[医药卫生—病原生物学]