一种新的藻红蛋白的亚基组成分析  

Analysis on subunit composition of a new phycoerythrin

作　　者：王盛[1] 钟伏弟[1] 吴祖建[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2004年第1期68-71,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技厅重大资助项目(2000H004;2001H127).

摘　　要：经盐析、离子交换层析、羟基磷灰石吸附层析和凝胶过滤层析等分离纯化,从珊瑚藻中分离纯化出R-藻红蛋白(R-PE).对其分子质量和亚基组成进行研究,结果表明:R-PE的分子质量为194ku,而目前报道的R-藻红蛋白分子质量为240ku;α、β亚基分子质量均为20ku左右,γ亚基分子质量约为31ku,推测的亚基组成为(αβ)4γ[不同于其他红藻中(αβ)6γ的藻红蛋白组成].By using fraction precipitation methods such as ammonium sulfate, ion exchanging, modified hydrorylapatite and gel filtration chromatography, a kind of phycoerythrin (R-PE) from Corallina officinalis was purified. The results of physical and chemical characteristics showed that the molecular mass of R-PE was 194 ku, which had been reported to be 240 ku; the molecular masses of α and β subunits were about 20 ku; the molecular mass of γ subunit was about 31 ku; subunit composition was not (αβ)_(6γ) (in other kinds of phycoerythrins) but (αβ)_(4γ) presumably.

关 键 词：R-藻红蛋白 亚基组成 PE 羟基磷灰石 分离纯化 珊瑚藻 左右 红藻 凝胶过滤层析 离子交换层析 

分 类 号：S512.1[农业科学—作物学] Q949.242[生物学—植物学]