慢性乙型重型肝炎患者血清乙型肝炎病毒基因型分析  

Analysis of hepatits B virus genotype in sera of chronic serious hepatitis B

作　　者：刘成永 梅存金 钱秀梅 侯远沛 宋丽 孙庆 

机构地区：[1]江苏省徐州市肝病研究所中心实验室,221004 [2]江苏省徐州市肝病研究所肝病科,221004

出　　处：《实用肝脏病杂志》2004年第4期198-199,202,共3页Journal of Practical Hepatology

摘　　要：目的　探讨慢性重型肝炎患者血清乙型肝炎病毒基因型特点及其与病毒复制水平的关系。方法　选择慢性肝炎、肝硬化和重型肝炎各 40例 ,用PCR微板核酸 ELISA杂交技术进行HBVDNA基因分型 ,并同时进行HBVDNA定量检测。结果　慢性重型肝炎组与肝硬化组D基因型和与D型相关的混合型所占百分率分别为 5 0 .0 %和 2 5 .0 % ,两者有显著差异 (x2 =4.3 ,P =0 .0 3 77) ;5 6.5 %的D基因型是慢性重型肝炎 ,而C基因型患者只有 2 8.3 % ,差异显著 (x2 =4.3 4,P =0 .0 3 7)。结论　乙型肝炎病毒基因型是否与慢性重型肝炎的发病有关 。ObjectiveTo study the HBV genotype of chronic serious hepatitis B and its relationship to the level of HBVDNA.MethodsHBV genotype was determined by PCR-microplate sandwich hybridization-ELISA technique in 120 HBVDNA positive patients including 40 chronic hepatitis B (CHB), 40 liver cirrhosis(LC) and 40 chronic serious hepatitis B And the value of HBVDNA was proceeded at the same time.ResultsThe percentage of genotype D and the mixed genotype associated with D was 50.0% in the group of chronic serious hepatitis B, significantly higher than 25.0% in 40 liver cirrhosis(x 2=4.32,P=0.037); 56.5% of the patients with genotype D had chronic serious hepatitis B and only 28.3% with genotype C had chronic serious hepatitis B. There was a significant difference(x 2=4.32,P=0.037).ConclusionThe examination of HBV genotype is a valid clinical means in the judgement of chronic serious hepatitis B,and do the help to the analysis of condition and trend against hepatitis B.

关 键 词：乙型肝炎病毒基因型 患者 慢性重型肝炎 血清 慢性乙型重型肝炎 肝硬化 HBVDNA定量 核酸 基因分型 杂交技术 

分 类 号：R512.62[医药卫生—内科学] R373[医药卫生—临床医学]