多重PCR技术检测医院感染大肠埃希菌、肺炎克雷伯菌质粒AmpC酶基因型别  

Detection of Plasmid-mediated AmpC β-Lactamase Gene from Clincal Isolates of Klebsiella pneumoniae and Escherichia coli by Using Multiplex PCR

作　　者：多丽波[1] 王孟滨[1] 程欣弘[1] 刘晶[1] 郭薇媛[1] 周虹[2] 

机构地区：[1]哈尔滨医科大学附属二院,黑龙江哈尔滨150086 [2]北京军事医学科学院野战输血研究所,北京100850

出　　处：《中华医院感染学杂志》2004年第10期1094-1097,共4页Chinese Journal of Nosocomiology

基　　金：黑龙江省卫生厅项目(编号 2 0 0 3 0 6 2 );哈尔滨市科学研究基金项目(编号 2 0 0 3AFXXJ0 4 5 )

摘　　要：目的　了解我地区大肠埃希菌、肺炎克雷伯菌携带质粒介导AmpC酶的情况及基因型别。方法　用头孢西丁耐药表型筛选阳性可疑菌株 ,提取耐药质粒 ,然后采用多重PCR技术检测是否存在质粒介导的AmpC基因及其基因型别 ;并对PCR产物进行测序。结果　 7株头孢西丁耐药的大肠埃希菌多重PCR均为阴性 ,4株肺炎克雷伯菌中 3株多重PCR阳性 ,测序结果均为DHA 1型质粒AmpC酶。结论　多重PCR技术是一种灵敏、特异、快速的检测质粒AmpC酶及其基因型别的好方法 ;采用该方法在我地区首次检测出产DHAOBJECTIVE To detect plasmid mediated AmpC β lactamase genes from clinical isolates of Klebsiella pneumoniae and Escherichia coli in Harbin city. METHODS Using multiplex PCR technique to amplify AmpC β lactamase gene and differentiate genotypes after extracted plasmid from cefoxitin resistant clinical strains, sequenced PCR products. RESULTS Three of 4 K. pneumoniae isolates produced DHA 1 AmpC β lactamases, and all seven E. coli isolates PCR were negative. CONCLUSIONS Multiplex PCR technique is a sensitive, specific and quick method for detecting plasmid mediated AmpC β lactamase gene; DHA 1 type plasmid mediated AmpC β lactamase gene is first discovered in K. pneumoniae isolates in our area.

关 键 词：质粒AMPC酶 多重PCR 大肠埃希菌 肺炎克雷伯菌 

分 类 号：R378.2[医药卫生—病原生物学]