聚合酶链反应检测绵羊肺炎霉形体的研究  

Development of polymerase chain reaction for detection of Mycoplasma ovipneumoniae in sheep

作　　者：张莉[1] 杨壮省 王英珍[1] 鄢明华[1] 孙继国[3] 

机构地区：[1]天津市畜牧兽医研究所,天津031120 [2]邢台市畜牧局,河北邢台054001 [3]河北农业大学,河北保定071001

出　　处：《中国兽医科技》2004年第11期7-10,共4页Chinese Journal of Veterinary Science and Technology

基　　金：河北省科技厅科技攻关项目 ( 0 2 2 2 0 4 0 6 )

摘　　要：参照基因库中已发表的绵羊肺炎霉形体Y98株的 1 6SrDNA序列 ,设计合成了 1对引物 ,建立了聚合酶链反应 (PCR)检测绵羊肺炎霉形体的方法。结果显示 ,所建立的PCR能特异扩增绵羊肺炎霉形体的DNA ,而对照的菌株均为阴性 ;其敏感性可达 1pg。经对绵羊肺炎霉形体分离株HD 1的扩增产物进行测序 ,并与绵羊肺炎霉形体标准株Y98的 1 6SrDNA序列进行比较 ,发现有A PCR method was developed with two oligonucleotide primers(ZW-5 and ZW-3 ), which were designed on the base of 16 S rDNA gene of M.ovipneumoniae standard strain Y98. Only a target DNA fragment was amplified from M.ovipneumoniaeDNA by the PCR. The sensitivityof the PCR technique was 1 pg.The amplified HD-1 strain DNA fragment was compared with 16 S rDNA part sequence of Y98 strain post-(sequencing). The results showed that these were six base’ differences between them.

关 键 词：绵羊肺炎霉形体 聚合酶链反应 16SrDNA 

分 类 号：S852.62[农业科学—基础兽医学]