压电基因传感器在HCMV链置换扩增实时检测中的实验研究  

Detecting HCMV Strand Displacement Amplifications by Piezoelectric Gene Sensor: An Experimental Research

作　　者：陈庆海[1] 府伟灵[1] 陈鸣[1] 张波[1] 汤万里[2] 蒋天伦[1] 公衍文[1] 蔡国儒[3] 

机构地区：[1]第三军医大学西南医院,重庆400038 [2]中国嘉陵集团生物工程开发部,重庆400032 [3]信息产业部电子第26研究所,重庆400060

出　　处：《中华医院感染学杂志》2004年第1期15-18,共4页Chinese Journal of Nosocomiology

基　　金：国家"863"计划(2002AARZ2023);国家"九五"科技攻关项目(96-A23-04-04);国家自然科学基金(30270388);军队"九五"课题(98MO940);军队"十五"课题(01MA180;01LO065);重庆市应用基础研究项目(2002-10-78);重庆市科委风险投资基金(0499405)

摘　　要：目的　探讨自行研发的压电基因传感器对链置换扩增 (SDA)的实时检测。方法　以巨细胞病毒 (HCMV)为检测对象 ,建立成熟的 SDA反应系统 ;在自行研发的压电基因传感器检测仪上对巨细胞病毒 SDA反应进行实时检测。结果　 SDA反应可引起压电基因传感器的频率持续下降 ;在一定范围内核酸杂交所引起的频率下降幅度与加入的靶序列浓度呈正相关 ;加样时温差等因素对早期频率变化曲线的影响不可忽视 ;实验温度是影响SDA压电基因传感器检测灵敏度的重要因素。结论　自行研发的压电基因传感器可成功实现对 SDA反应的实时检测 ;该系统可直接检测基因组 DNA并可广泛应用于病原微生物的临床检测。OBJECTIVETo detect strand displacement amplifications (SDA) by piezoelectric gene sensor made by ourselves. METHODSTo set up maid SDA system by detecting human cytomegalovirus (HCMV) and detect SDA of HCMV by piezoelectric gene sensor. RESULTSSDA could lead the continuous reduction of frequency in piezoelectric gene sensor. The degree of frequency reduction leaded by gene reaction was dependent on consistency of the objective gene in limited conditions. It cannot be neglected the effect of the different temperature between the two liquids on primary frequency. Test temperature is important to effect the sensitivity of the SDA piezoelectric gene sensor. CONCLUSIONSThe piezoelectric gene sensor made by ourselves could detect SDA successfully. The system can directly detect gene DNA and be used in the clinical detecting of harmful microbiology extensively.

关 键 词：压电基因传感器 链置换扩增 巨细胞病毒 

分 类 号：R446.1[医药卫生—诊断学]