链置换扩增压电DNA传感器检测铜绿假单胞菌的临床应用研究  

Detection of Pseudomonas aeruginosaby SDA Piezoelectric DNA Sensors Array: A Clinical Application

作　　者：陈斌[1] 府伟灵[1] 蒋天伦[1] 黄君富[1] 

机构地区：[1]第三军医大学西南医院,重庆400038

出　　处：《中华医院感染学杂志》2004年第1期22-24,共3页Chinese Journal of Nosocomiology

基　　金：国家"863"计划(2002AARZ2023);国家"九五"科技攻关项目(96-A23-04-04);国家自然科学基金(30270388);军队"九五"课题(98MO94);军队"十五"课题(01MA180;01LO065)

摘　　要：目的　探讨链置换扩增 (SDA )压电 DNA传感器检测铜绿假单胞菌的临床应用。方法　应用 SDA压电DNA传感器芯片技术和特异性寡核苷酸探针 ,对临床标本 33份进行铜绿假单胞菌检测 ,并以荧光定量 PCR作参照 ,与常规培养进行对比分析。结果　在 33份临床标本中 ,SDA压电 DNA传感器检出 17份铜绿假单胞菌阳性 ,与荧光定量 PCR结果完全一致 ,而这两种方法与常规分离培养略有差异。结论　 SDA压电 DNA传感器芯片技术能直接检测铜绿假单胞菌基因组 DNA,具有准确、快速、灵敏的优点。OBJECTIVE To detect Pseudomonas aeruginosa in clinical samples with SDA piezoelectric DNA sensors array. METHODS Technique of SDA piezoelectric DNA sensors array was used to detect P. aeruginosa in 33 clinical samples and the results were compared with those obtained by fluorescence quantitative PCR (FQ-PCR) and conventional cultivation.RESULTS The detection results of SDA piezoelectric DNA sensors array were identical to those of FQ-PCR, but had some difference with those of conventional cultivation. Among 33 clinical samples, 17 samples showed positive of P. aeruginosa detected by sensor method.CONCLUSIONS SDA piezoelectric DNA sensors array is a rapid and specific method for detection of the genomic DNA for P. aeruginosa.

关 键 词：链置换扩增 压电DNA传感器 铜绿假单胞菌 

分 类 号：R446.1[医药卫生—诊断学]